Metabolomic analysis shows differential hepatic effects of T2 and T3 in rats after short-term feeding with high fat diet

Scientific Reports
Liliana F IannucciRohit A Sinha

Abstract

Nonalcoholic fatty liver disease (NAFLD) is a major health problem worldwide, and is often associated with lipotoxic injury, defective mitochondrial function, and insulin resistance. Thyroid hormones (THs) are important regulators of hepatic lipid metabolism. Among the THs, diiodothyronine (T2) and triiodothyronine (T3) have shown promising results in lowering hepatic fat content in various models of NAFLD. In this study, we used a targeted metabolomics approach to investigate the differential effects of T2 and T3 on the early metabolic adaptation in the livers of rats fed high fat diet (HFD), a period when hepatosteatosis is reversible. Our results showed that both T2 and T3 strongly induced autophagy and intra-hepatic acylcarnitine flux but prevented the generation of sphingolipid/ceramides in animals fed HFD. Interestingly, although both T2 and T3 decreased hepatic fat content, only T2 was able to rescue the impairment in AKT and MAPK/ERK pathways caused by HFD. In summary, we have identified and characterized the effects of T2 and T3 on hepatic metabolism during short-term exposure to HFD. These findings illuminate the common and divergent metabolic pathways by T2 and T3 that also may be important in the prevention and trea...Continue Reading

References

Jun 13, 1994·FEBS Letters·F GogliaB Kadenbach
May 29, 2004·The Journal of Biological Chemistry·Varman T SamuelGerald I Shulman
Jul 15, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Antonia LanniFernando Goglia
Apr 12, 2007·Obesity·Roland BuettnerL Cornelius Bollheimer
Dec 17, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Edward E CableMark D Erion
Apr 3, 2009·Nature·Rajat SinghMark J Czaja
Jan 7, 2010·Endocrine Reviews·Sheue-Yann ChengPaul J Davis
Apr 7, 2010·Clinical Lipidology·Sophie C Cazanave, Gregory J Gores
Aug 5, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Brent A Neuschwander-Tetri
Oct 23, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kuok Teong OngDouglas G Mashek
Dec 15, 2010·Journal of Hepatology·Elena GrasselliLaura Vergani
Jun 7, 2011·Cell Metabolism·Maryam AhmadianHei Sook Sul
Jun 15, 2011·Molecular and Cellular Endocrinology·Joachim M Weitzel, K Alexander Iwen
Nov 24, 2011·The Journal of Endocrinology·Elena GrasselliLaura Vergani
Jun 12, 2012·The Journal of Clinical Investigation·Rohit Anthony SinhaPaul M Yen
Dec 22, 2012·Diabetes·Marieke G SchoonemanMaarten R Soeters
Apr 23, 2013·Nature Cell Biology·Carmine SettembreAndrea Ballabio
May 9, 2013·American Journal of Physiology. Endocrinology and Metabolism·Daniel F VatnerVarman T Samuel
Jun 19, 2013·Advances in Experimental Medicine and Biology·Mahua Chakraborty, Xian-Cheng Jiang
Aug 10, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rohit A SinhaPaul M Yen
Aug 21, 2013·Biochimica Et Biophysica Acta·Alessio RochiraLuisa Siculella
Aug 24, 2013·Endocrine Reviews·Einav Yehuda-ShnaidmanJacob Bar-Tana
Jan 28, 2014·The Journal of Endocrinology·Rosalba SeneseAntonia Lanni
Feb 4, 2014·Liver International : Official Journal of the International Association for the Study of the Liver·Witigo von SchönfelsJochen Hampe
May 27, 2014·Archives of Biochemistry and Biophysics·Yoshihiko TerashimaMasaru Yoshida
Aug 17, 2014·Trends in Endocrinology and Metabolism : TEM·Rohit A SinhaPaul M Yen
Sep 19, 2014·Biochemical Society Transactions·Ainara Cano, Cristina Alonso
Dec 30, 2014·Frontiers in Physiology·Rosalba SenesePieter de Lange
Jun 11, 2015·American Journal of Physiology. Endocrinology and Metabolism·Nishanth E SunnyKenneth Cusi
Sep 29, 2015·Trends in Endocrinology and Metabolism : TEM·Bhagirath Chaurasia, Scott A Summers
Mar 22, 2016·Metabolism: Clinical and Experimental·Reenam S Khan, Philip N Newsome
Aug 4, 2016·Journal of Hepatology·Raluca PaisFilomena Conti
Oct 25, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jari E KaikkonenOlli T Raitakari

❮ Previous
Next ❯

Citations

Feb 24, 2018·Nature Reviews. Endocrinology·Rohit A SinhaPaul M Yen
Jan 10, 2019·Annual Review of Pharmacology and Toxicology·Leigh GoedekeGerald I Shulman
Sep 27, 2019·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·Jia LiuGuang Wang
Jul 26, 2018·Frontiers in Endocrinology·Marta A KowalikAndrea Perra
Aug 8, 2019·Thyroid : Official Journal of the American Thyroid Association·Rohit A SinhaPaul M Yen
Apr 29, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Megan J RitterAnthony N Hollenberg
Apr 4, 2019·BioMed Research International·Ying WangHaoming Luo
Jul 31, 2019·Endocrine·Rosalba SeneseAntonia Lanni
Jun 14, 2020·International Journal of Molecular Sciences·Marco GiammancoItalia Di Liegro
Aug 23, 2020·International Journal of Molecular Sciences·Daniela Maria TanaseMariana Floria
Aug 10, 2018·Frontiers in Endocrinology·Rosalba SeneseAntonia Lanni
Nov 14, 2019·Scientific Reports·Rosalba SeneseNicoletta Potenza
Apr 25, 2020·Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association·G Sebastian HönesLars C Moeller
May 21, 2018·Biochemical and Biophysical Research Communications·David J MontefuscoL Ashley Cowart
Jul 2, 2019·European Thyroid Journal·Josef Köhrle

❮ Previous
Next ❯

Methods Mentioned

BETA
metabolomic profiling

Software Mentioned

ImageJ
Agilent MassHunter Workstation

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Related Papers

Hepatology Research : the Official Journal of the Japan Society of Hepatology
Lisa LongatoSuzanne M de la Monte
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
Hui-Min SuQiong Zhang
Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Rohit A SinhaPaul M Yen
© 2022 Meta ULC. All rights reserved