Metabolomic and proteomic responses of Staphylococcus aureus to prolonged cold stress

Journal of Proteomics
Mousa M AlreshidiTim K Roberts

Abstract

The high pathogenicity of Staphylococcus aureus is thought to be due to its extraordinary capacity to rapidly adapt to changes in environmental conditions. This study was carried out to investigate whether the cytoplasmic profiles of metabolites and proteins of S. aureus were altered in response to prolonged exposure to cold stress. Metabolic profiling and proteomics were used to characterise alterations in cytoplasmic proteins and metabolites in cells from the mid-exponential phase of growth under ideal conditions at 37°C and compared with equivalent cells exposed to prolonged cold stress for 2 weeks at 4°C. Principle component analysis (PCA) of the metabolomic and proteomic data indicated that, at the mid-exponential phase of growth, prolonged cold stress conditions generated cells with different metabolite and protein profiles compared with those grown at 37°C. Nine ribosomal proteins and citric acid were substantially elevated in the cytoplasmic fractions from the cells adapted to cold-stress but most amino acids showed a reduction in their concentration in cold-stressed samples. The data provided strong evidence supporting the hypothesis that specific changes in metabolic homeostasis and protein composition were critical t...Continue Reading

References

Oct 6, 2000·Annual Review of Microbiology·C L Squires, D Zaporojets
Oct 16, 2001·Australian Veterinary Journal·G K BrownR J Aitken
Jan 14, 2003·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Nicole BaumertHans-Georg Sahl
Aug 7, 2003·Trends in Genetics : TIG·Constance J Jeffery
Aug 17, 2004·Science·Nathalie Q BalabanStanislas Leibler
Oct 8, 2004·Molecular Microbiology·Delfina C Dominguez
Feb 1, 2005·Trends in Microbiology·Kaarina LähteenmäkiTimo K Korhonen
Sep 12, 2006·Biochimica Et Biophysica Acta·Irina BrandinaRobert P Martin
Nov 9, 2006·Archives of Dermatological Research·Lars Alexander SchneiderJoachim Dissemond
Jan 9, 2008·International Journal of Antimicrobial Agents·Christof von Eiff
Sep 23, 2008·Journal of Basic Microbiology·Palas Kumar ChandaSubrata Sau
Jan 24, 2009·Trends in Microbiology·Parham Sendi, Richard A Proctor
Apr 28, 2009·Molecular BioSystems·Constance J Jeffery
Dec 17, 2009·International Journal of Medical Microbiology : IJMM·Michael HeckerSusanne Engelmann
Dec 17, 2009·Journal of Experimental Botany·Chen-Chi WuLaurent Zimmerli
Feb 13, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Luay F Abu-QatousehKarsten Becker
Oct 19, 2010·International Journal of Food Microbiology·B SánchezJ J R Herrera
Mar 16, 2011·International Journal of Food Microbiology·Sinisa VidovicDarren R Korber
May 20, 2011·Proteomics·André KriegeskorteKarsten Becker
Mar 8, 2012·Molekuliarnaia biologiia·L V Aseev, I V Boni
Jul 24, 2012·Biochemistry. Biokhimii︠a︡·A V KorobeinikovaG M Gongadze
Apr 24, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Alanna SmithKevin Kavanagh

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Citations

Feb 9, 2016·Critical Reviews in Food Science and Nutrition·Akanksha Gandhi, Nagendra P Shah
Oct 18, 2018·Journal of Pathogens·Laura A Onyango, Mousa M Alreshidi
Jul 2, 2020·Infection and Drug Resistance·Ehsaneh KhodadadiHossein Samadi Kafil
Aug 6, 2017·Scientific Reports·Phan Nguyen Thuy AnMasamitsu Yamaguchi
Dec 24, 2018·Frontiers in Microbiology·Xiaojing TianRuitong Dai
Sep 3, 2019·Frontiers in Microbiology·Carlo R CarereMatthew B Stott
Dec 4, 2017·Metabolomics : Official Journal of the Metabolomic Society·Cyril JousseAnne-Marie Delort
Mar 5, 2021·Nucleic Acids Research·Arancha Catalan-MorenoAlejandro Toledo-Arana
Jun 3, 2016·Journal of Proteome Research·Zhe WangXuan-Xian Peng

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