Metabolomic and transcriptomic changes underlying cold and anaerobic stresses after storage of table grapes

Scientific Reports
Itay MaozAmnon Lichter

Abstract

The currently accepted paradigm is that fruits and vegetables should be consumed fresh and that their quality deteriorates during storage; however, there are indications that some metabolic properties can, in fact, be improved. We examined the effects of low temperature and high-CO2 conditions on table grapes, Vitis vinifera L. cv. 'Superior Seedless'. Berries were sampled at harvest (T0) and after low-temperature storage for 6 weeks under either normal atmosphere conditions (TC) or under an O2 level of 5 kPa and elevated CO2 levels of 5, 10 or 15 kPa (T5, T10, T15). Accumulation of 10 stilbenes, including E-ε-viniferin, E-miyabenol C and piceatannol, significantly increased under TC treatment as compared to T0 or T15. Sensory analysis demonstrated that elevated CO2 elicited dose-dependent off-flavor accumulation. These changes were accompanied by an accumulation of 12 volatile metabolites, e.g., ethyl acetate and diacetyl, that imparted disagreeable flavors to fresh fruit. Transcriptome analysis revealed enrichment of genes involved in pyruvate metabolism and the phenylpropanoid pathway. One of the transcription factors induced at low temperature but not under high CO2 was VvMYB14, which regulates stilbene biosynthesis. Our fi...Continue Reading

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Citations

May 14, 2020·Metabolites·Delphine M PottSonia Osorio
Dec 11, 2020·International Journal of Molecular Sciences·Irene RomeroMaria Teresa Sanchez-Ballesta
Mar 23, 2021·Frontiers in Plant Science·Crista Ann BurbidgeCrystal Sweetman
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·James W FavellWesley F Zandberg
Jun 10, 2020·Journal of Agricultural and Food Chemistry·Guodong CaoZongwei Cai

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Methods Mentioned

BETA
RNAseq
transgenic

Software Mentioned

PANTHER
JMP
Cluster
agriGO
trimmomatic
MetGenMap
MassHunter
Venny
Bowtie
Treeview

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