Metabolomic approach for improving ethanol stress tolerance in Saccharomyces cerevisiae

Journal of Bioscience and Bioengineering
Erika OhtaEiichiro Fukusaki

Abstract

The budding yeast Saccharomyces cerevisiae is widely used for brewing and ethanol production. The ethanol sensitivity of yeast cells is still a serious problem during ethanol fermentation, and a variety of genetic approaches (e.g., random mutant screening under selective pressure of ethanol) have been developed to improve ethanol tolerance. In this study, we developed a strategy for improving ethanol tolerance of yeast cells based on metabolomics as a high-resolution quantitative phenotypic analysis. We performed gas chromatography-mass spectrometry analysis to identify and quantify 36 compounds on 14 mutant strains including knockout strains for transcription factor and metabolic enzyme genes. A strong relation between metabolome of these mutants and their ethanol tolerance was observed. Data mining of the metabolomic analysis showed that several compounds (such as trehalose, valine, inositol and proline) contributed highly to ethanol tolerance. Our approach successfully detected well-known ethanol stress related metabolites such as trehalose and proline thus, to further prove our strategy, we focused on valine and inositol as the most promising target metabolites in our study. Our results show that simultaneous deletion of LE...Continue Reading

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Citations

Aug 24, 2017·FEMS Microbiology Reviews·Maria C DzialoKevin J Verstrepen
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Honglun YuanShouwen Chen
Oct 18, 2019·PloS One·Amaury PupoJennifer E G Gallagher
Mar 23, 2018·Digestive Diseases and Sciences·Shin NishiumiMasaru Yoshida
Jan 3, 2019·Antonie van Leeuwenhoek·Mariana Caroline Tocantins AlvimWendel Batista da Silveira
Mar 12, 2020·Frontiers in Bioengineering and Biotechnology·Luan Luong ChuHanhong Bae
Jul 18, 2020·Applied Microbiology and Biotechnology·Fernando Augusto da SilveiraWendel Batista da Silveira
Sep 21, 2019·Journal of Bioscience and Bioengineering·Makoto ImuraEiichiro Fukusaki
Jul 9, 2021·Journal of Proteome Research·Nikolina ŠoštarićVera van Noort

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