Metabolomic Fingerprints of Individual Algal Cells Using the Single-Probe Mass Spectrometry Technique

Frontiers in Plant Science
Mei SunBoris Wawrik

Abstract

Traditional approaches for the assessment of physiological responses of microbes in the environment rely on bulk filtration techniques that obscure differences among populations as well as among individual cells. Here, were report on the development on a novel micro-scale sampling device, referred to as the "Single-probe," which allows direct extraction of metabolites from living, individual phytoplankton cells for mass spectrometry (MS) analysis. The Single-probe is composed of dual-bore quartz tubing which is pulled using a laser pipette puller and fused to a silica capillary and a nano-ESI. For this study, we applied Single-probe MS technology to the marine dinoflagellate Scrippsiella trochoidea, assaying cells grown under different illumination levels and under nitrogen (N) limiting conditions as a proof of concept for the technology. In both experiments, significant differences in the cellular metabolome of individual cells could readily be identified, though the vast majority of detected metabolites could not be assigned to KEGG pathways. Using the same approach, significant changes in cellular lipid complements were observed, with individual lipids being both up- and down-regulated under light vs. dark conditions. Conver...Continue Reading

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Citations

May 10, 2019·Marine Drugs·Chiara LauritanoAlessandra Rogato
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Dec 26, 2018·Analytical Chemistry·Mei Sun, Zhibo Yang

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Methods Mentioned

BETA
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Software Mentioned

Metaboanalyst
Pathos
LabView
PLS
DA
METLIN
Geena
Zenodo

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