Metabolomic Profiling of Mice Serum during Toxoplasmosis Progression Using Liquid Chromatography-Mass Spectrometry

Scientific Reports
Chun-Xue ZhouXing-Quan Zhu

Abstract

Better understanding of the molecular changes associated with disease is essential for identifying new routes to improved therapeutics and diagnostic tests. The aim of this study was to investigate the dynamic changes in the metabolic profile of mouse sera during T. gondii infection. We carried out untargeted metabolomic analysis of sera collected from female BALB/c mice experimentally infected with the T. gondii Pru strain (Genotype II). Serum samples were collected at 7, 14 and 21 day post infection (DPI) from infected and control mice and were subjected to liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS)-based global metabolomics analysis. Multivariate statistical analysis identified 79 differentially expressed metabolites in ESI + mode and 74 in ESI - mode in sera of T. gondii-infected mice compared to the control mice. Further principal component analysis (PCA) and partial least squares-discrimination analysis (PLS-DA) identified 19 dysregulated metabolites (5 in ESI + mode and 14 in ESI - mode) related to the metabolism of amino acids and energy metabolism. The potential utility of these metabolites as diagnostic biomarkers was validated through receiver operating characteristic (ROC) curve ...Continue Reading

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Citations

Jan 15, 2017·Molecular & Cellular Proteomics : MCP·Zhaoshou YangXingwang Zhou
Sep 21, 2020·Parasitology Research·Yu-Meng MengJun-Jun He
Apr 5, 2016·Metabolic Brain Disease·Hany M ElsheikhaXing-Quan Zhu
Feb 15, 2018·Infection and Immunity·Guangping HuangXianyong Liu
Jun 21, 2018·Frontiers in Cellular and Infection Microbiology·Xiao-Qing ChenXing-Quan Zhu
Jun 28, 2019·Frontiers in Cellular and Infection Microbiology·Stephanie J Melchor, Sarah E Ewald
Jun 28, 2021·Parasites & Vectors·Xue-Mei WuGuang-Hui Zhao
Mar 16, 2017·Environmental Science & Technology·Zhihao YuBrian H Clowers

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Methods Mentioned

BETA
nuclear magnetic resonance
PCA
PCR

Software Mentioned

R
SIMCA
DA
XCMS
OPLS
- P +
MetaboloAnalyst
MetaboAnalyst

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