Metabolomic profiling of regulatory lipid mediators in sputum from adult cystic fibrosis patients.

Free Radical Biology & Medicine
Jun YangBruce D Hammock

Abstract

Retained respiratory tract (RT) secretions, infection, and exuberant inflammatory responses are core abnormalities in cystic fibrosis (CF) lung disease. Factors contributing to the destructive CF airway inflammatory processes remain incompletely characterized. The pro-oxidative inflammatory CF RT milieu is known to contain enzymatically and nonenzymatically produced regulatory lipid mediators, a panel of structurally defined oxidized metabolites of polyunsaturated fatty acids known to play a role in pathology related to inflammation. Using an extraction protocol that maximizes recoveries of sputum-spiked deuterated standards, coupled with an LC/MS/MS detection system, this study presents a metabolomic method to assess a broad spectrum of regulatory lipid mediators in freshly obtained sputum from CF patients. A broad range of both proinflammatory and anti-inflammatory lipid mediators was detected, including PGE2, PGD2, TXB2, LTB4, 6-trans-LTB4, 20-OH-LTB4, 20-COOH-LTB4, 20-HETE, 15-HETE, 11-HETE, 12-HETE, 8-HETE, 9-HETE, 5-HETE, EpETrEs, diols, resolvin E1, 15-deoxy-PGJ2, and LXA4. The vast majority of these oxylipins have not been reported previously in CF RT secretions. Whereas direct associations of individual proinflammatory...Continue Reading

References

Feb 17, 1978·Science·W J VaughanS Abraham
Dec 1, 1990·British Journal of Clinical Pharmacology·A P SampsonJ F Price
Sep 1, 1987·The American Review of Respiratory Disease·J T ZakrzewskiP J Piper
Jan 1, 1987·British Journal of Clinical Pharmacology·J T ZakrzewskiJ F Costello
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J Carlstedt-DukeB Strandvik
Mar 30, 1995·The New England Journal of Medicine·M W KonstanP B Davis
May 1, 1997·Nature Medicine·M F MoghaddamB D Hammock
Aug 7, 2002·Clinical Reviews in Allergy & Immunology·James F ChmielMichael W Konstan
Feb 6, 2004·The New England Journal of Medicine·Steven D FreedmanBrian P O'Sullivan
Feb 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Russell E VanceJohn J Mekalanos
Mar 23, 2004·Nature Immunology·Christopher L KarpNicos A Petasis
Mar 31, 2004·Nature Immunology·Daiya TakaiTakao Shimizu
Sep 24, 2004·American Journal of Respiratory Cell and Molecular Biology·David R KoehlerJim Hu
Sep 25, 2004·Molecular Microbiology·Hiromi Sato, Dara W Frank
Sep 25, 2004·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Erik J SaudeRedwan Moqbel
Mar 1, 2005·American Journal of Respiratory and Critical Care Medicine·Tricia M McKeeverPatricia A Cassano
May 13, 2005·The New England Journal of Medicine·Steven M RoweEric J Sorscher
Jun 23, 2005·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Harry Heijerman
Sep 17, 2005·American Journal of Respiratory and Critical Care Medicine·Andrew J SloaneJenny L Harry
Nov 29, 2005·Cellular Microbiology·A M SalibaM C Plotkowski
Apr 11, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Gye Young Park, John W Christman
Jun 30, 2006·The European Respiratory Journal·V StarostaM Griese
Jul 11, 2006·American Journal of Physiology. Cell Physiology·Terry E Machen
Sep 23, 2006·American Journal of Respiratory Cell and Molecular Biology·Caroline Bonnans, Bruce D Levy
Jan 9, 2007·Respirology : Official Journal of the Asian Pacific Society of Respirology·David W ReidE Haydn Walters
Jul 27, 2007·Proceedings of the American Thoracic Society·Scott D SagelMichael W Konstan
Sep 18, 2007·Journal of Bacteriology·Kelli L PalmerMarvin Whiteley
Nov 27, 2007·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M Rabie Al-TurkmaniMichael Laposata
Feb 16, 2008·Prostaglandins, Leukotrienes, and Essential Fatty Acids·S Van BiervlietA Christophe
Mar 12, 2008·Molecular Interventions·Karsten Gronert
Apr 4, 2008·The European Respiratory Journal·J LiW-Y Lu
Sep 25, 2008·Current Opinion in Pulmonary Medicine·Joseph M Collaco, Garry R Cutting
Dec 11, 2008·Cellular Microbiology·Gerd Döring, Erich Gulbins
Mar 14, 2009·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Justyna E WolakThomas M O'Connell
Jun 9, 2009·American Journal of Respiratory Cell and Molecular Biology·Gerrit JohnMarkus O Henke
Jul 10, 2009·The American Journal of Clinical Nutrition·Luigi IulianoSerena Quattrucci
Sep 15, 2009·Drugs·Andrew M Jones, Jennifer M Helm
Oct 31, 2009·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Bronwen RhodesAnne Stephenson
Jan 14, 2010·European Journal of Human Genetics : EJHG·Kathryn E McDougalGarry R Cutting
Feb 5, 2010·Mucosal Immunology·A PlanagumàB D Levy
May 7, 2010·Metabolomics : Official Journal of the Metabolomic Society·Rima Kaddurah-DaoukRonald M Krauss

❮ Previous
Next ❯

Citations

Jun 25, 2013·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Suha JabrCésar Picado
Jan 23, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Carolina H Pohl, Johan L F Kock
Oct 11, 2012·Bioanalysis·Stuart SnowdenCraig E Wheelock
Jan 21, 2014·Annals of the American Thoracic Society·Alison Morris
Aug 16, 2014·Journal of Chromatography. a·Julia HomannNerea Ferreirós
Feb 28, 2013·Biochemistry·Stephen B MilneH Alex Brown
Jan 23, 2015·Chest·Charles R EstherRichard C Boucher
Nov 13, 2014·Analytical Chemistry·Caroline H JohnsonGary Siuzdak
Jun 9, 2015·Paediatric Respiratory Reviews·Kathryn A RamseyStephen M Stick
Dec 2, 2015·The ISME Journal·Robert A QuinnForest L Rohwer
Nov 8, 2015·European Journal of Pharmacology·Melody G Duvall, Bruce D Levy
Mar 3, 2015·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Jiangchao ZhaoJohn J LiPuma
Jul 5, 2012·Annals of the New York Academy of Sciences·Jason P EiserichCarroll E Cross
Jun 5, 2013·Molecular Nutrition & Food Research·Sharon A Murphy, Anna Nicolaou
Jul 13, 2013·Pediatric Pulmonology·Elizabeth JoseloffMarianne S Muhlebach
Jul 29, 2015·Current Opinion in Immunology·Benjamin J Marsland, Olawale Salami
Mar 18, 2015·American Journal of Respiratory Cell and Molecular Biology·Caroline MorinSamuel Fortin
Jul 24, 2014·Cell Biology International·Qing QuGuo-Huang Fan
Jun 27, 2015·Pediatric Pulmonology·Theresa A LagunaChris H Wendt
Sep 12, 2013·Redox Biology·Angel Gabriel Valdivieso, Tomás A Santa-Coloma
Apr 22, 2015·European Journal of Pharmacology·Mario RomanoAntonio Recchiuti
Nov 19, 2014·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·B Fatemeh Nobakht M GhAfsaneh Arefi Oskouie
Aug 31, 2014·Free Radical Biology & Medicine·Elizabeth J ReverriFrancene M Steinberg
Sep 4, 2012·Free Radical Biology & Medicine·Karen A Massey, Anna Nicolaou
Apr 24, 2013·The Journal of Allergy and Clinical Immunology·Shamsah KazaniElliot Israel
May 22, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Xianai WuHans-Joachim Lehmler
Oct 21, 2014·PloS One·Jennifer K FredianiThomas R Ziegler
Jul 19, 2016·Oral Diseases·S Sommakia, O J Baker
Dec 17, 2016·Proceedings of the National Academy of Sciences of the United States of America·Becca A FlitterJennifer M Bomberger
Apr 30, 2017·Molecular Aspects of Medicine·Melody G DuvallBruce D Levy
Oct 2, 2015·Electrophoresis·Paolo IadarolaSimona Viglio
Apr 7, 2018·Pediatric Pulmonology·Sophia KarandashovaJudith A Voynow
May 3, 2018·Physiological Reviews·Nandini KrishnamoorthyBruce D Levy
Jul 18, 2017·Pediatric Pulmonology·Olivia Giddings, Charles R Esther
Aug 4, 2018·Analytical and Bioanalytical Chemistry·Zhi-Xin YuanChristopher E Ramsden
Oct 5, 2017·Biomedical Chromatography : BMC·Rhishikesh ThakareYazen Alnouti
Jan 25, 2019·Journal of Leukocyte Biology·Anne-Sophie ArchambaultNicolas Flamand
Nov 12, 2016·The European Respiratory Journal·Charles R EstherUNKNOWN AREST CF
Nov 7, 2015·Molecular and Cellular Pediatrics·Marianne S Muhlebach, Wei Sha
Mar 29, 2019·Expert Opinion on Therapeutic Targets·Yuanyuan Cheng, Jianhui Rong
Dec 22, 2015·Nature Reviews. Immunology·Maria C Basil, Bruce D Levy

❮ Previous
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