Metabolomic profiling of tumor-infiltrating macrophages during tumor growth

Cancer Immunology, Immunotherapy : CII
Naoki UmemuraHiroshi Sakagami

Abstract

Myeloid-derived suppressor cells (MDSCs) and tumor-associated macrophages (TAMs) are both key immunosuppressive cells that contribute to tumor growth. Metabolism and immunity of tumors depend on the tumor microenvironment (TME). However, the intracellular metabolism of MDSCs and TAMs during tumor growth remains unclear. Here, we characterized CD11b+ cells isolated from a tumor-bearing mouse model to compare intratumoral TAMs and intrasplenic MDSCs. Intratumoral CD11b+ cells and intrasplenic CD11b+ cells were isolated from tumor-bearing mice at early and late stages (14 and 28 days post-cell transplantation, respectively). The cell number of intrasplenic CD11b+ significantly increased with tumor growth. These cells included neutrophils holding segmented leukocytes or monocytes with an oval nucleus and Gr-1hi IL-4Rαhi cells without immunosuppressive function against CD8 T cells. Thus, these cells were classified as MDSC-like cells (MDSC-LCs). Intratumoral CD11b+ cells included macrophages with a round nucleus and were F4/80hi Gr-1lo IL-4Rαhi cells. Early stage intratumoral CD11b+ cells inhibited CD8 T cells via TNFα. Thus, this cell population was classified as TAMs. Metabolomic analyses of intratumoral TAMs and MDSC-LCs during t...Continue Reading

References

Jun 18, 2003·Trends in Immunology·Vincenzo BrontePaola Zanovello
Jun 21, 2008·Journal of Leukocyte Biology·Kenichi NonakaTsuyoshi Takami
Oct 4, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Je-In YounDmitry I Gabrilovich
Apr 4, 2009·Nature Reviews. Cancer·Frances Balkwill
Dec 24, 2009·Cancer Research·Minu K SrivastavaSuzanne Ostrand-Rosenberg
Mar 20, 2010·Metabolomics : Official Journal of the Metabolomic Society·Masahiro SugimotoMasaru Tomita
Sep 30, 2010·The Journal of Experimental Medicine·Cesar A CorzoDmitry I Gabrilovich
Oct 20, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bingjiao YinPing-Ying Pan
Mar 1, 2012·Seminars in Cancer Biology·Paola FilipazziVeronica Huber
Mar 23, 2012·Current Bioinformatics·Masahiro SugimotoMasaru Tomita
May 10, 2013·Seminars in Immunopathology·Subhra K BiswasAlberto Mantovani
Jun 7, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michael ProsniakD Craig Hooper
Oct 31, 2013·Cancer Science·Yoshihiro KomoharaMotohiro Takeya
Nov 29, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Melisa J HamiltonKevin L Bennewith
Apr 30, 2014·The Journal of Experimental Medicine·Muhammad Zaeem NomanSalem Chouaib
Jun 27, 2014·Annals of the New York Academy of Sciences·Samantha SolitoVincenzo Bronte
Sep 10, 2014·Immunology·Vinit Kumar, Dmitry I Gabrilovich
Oct 24, 2014·Annual Review of Medicine·Thomas CondamineDmitry I Gabrilovich
Apr 29, 2015·Biomaterials·Rene Garcia-ContrerasHiroshi Sakagami
Jan 7, 2016·Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society·Masahiro Sugimoto
Jun 21, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·Koichi SakakuraKazuaki Chikamatsu
Jul 7, 2016·Nature Communications·Vincenzo BronteDmitry I Gabrilovich
Jul 12, 2016·Nature Reviews. Immunology·Luke A J O'NeillJeff Rathmell
Oct 25, 2016·Cell Metabolism·Mathias WenesMassimiliano Mazzone
Jan 24, 2017·Cell Metabolism·Eric H MaRussell G Jones
Feb 12, 2017·Cell·Matthew G Vander Heiden, Ralph J DeBerardinis
Mar 2, 2017·Proceedings of the National Academy of Sciences of the United States of America·Carlos Carmona-FontaineJoao B Xavier
Apr 4, 2017·Cancer Science·Toshimitsu OhashiYatsuji Ito
May 28, 2017·International Immunopharmacology·Rufei YuanXiaolin Yuan
Sep 26, 2017·Nature Reviews. Immunology·Glenn R BantugChristoph Hess
Mar 20, 2018·Frontiers in Immunology·Viktor FlemingViktor Umansky
Jun 13, 2018·Frontiers in Immunology·Yuqiao ShengZhihai Qin

❮ Previous
Next ❯

Methods Mentioned

BETA
Flow cytometry

Software Mentioned

GraphPad
CellQuest
MasterHands

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Cancer -Omics

A variety of different high-throughput technologies can be used to identify the complete catalog of changes that characterize the molecular profile of cohorts of tumor samples. Discover the latest insights gained from cancer 'omics' in this feed.