Metabolomic Response of Equine Skeletal Muscle to Acute Fatiguing Exercise and Training

Frontiers in Physiology
Dylan J KleinTracy G Anthony

Abstract

The athletic horse, despite being over 50% muscle mass, remains understudied with regard to the effects of exercise and training on skeletal muscle metabolism. To begin to address this knowledge gap, we employed an untargeted metabolomics approach to characterize the exercise-induced and fitness-related changes in the skeletal muscle of eight unconditioned Standardbred horses (four male, four female) before and after a 12-week training period. Before training, unconditioned horses showed a high degree of individual variation in the skeletal muscle metabolome, resulting in very few differences basally and at 3 and 24 h after acute fatiguing exercise. Training did not alter body composition but did improve maximal aerobic and running capacities (p < 0.05), and significantly altered the skeletal muscle metabolome (p < 0.05, q < 0.1). While sex independently influenced body composition and distance run following training (p < 0.05), sex did not affect the skeletal muscle metabolome. Exercise-induced metabolomic alterations (p < 0.05, q < 0.1) largely centered on the branched-chain amino acids (BCAA), xenobiotics, and a variety of lipid and nucleotide-related metabolites, particularly in the conditioned state. Further, training incr...Continue Reading

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Citations

Jun 9, 2020·Journal of Animal Physiology and Animal Nutrition·Dylan J KleinKenneth H McKeever
May 29, 2021·Scientific Reports·Hajime OhmuraToshiyuki Takahashi
Jun 29, 2021·American Journal of Physiology. Endocrinology and Metabolism·Juulia H LautaojaJuha J Hulmi
Oct 28, 2021·Scandinavian Journal of Medicine & Science in Sports·Alessandro M ZagattoAlex Castro

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Methods Mentioned

BETA
biopsies
blood draws
biopsy
PCA

Software Mentioned

SigmaStat
Primer3
Metabolon
OpenLab
R

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