Metabolomic response of osteosarcoma cells to nanographene oxide-mediated hyperthermia.

Materials Science & Engineering. C, Materials for Biological Applications
Mónica CicuéndezIola F Duarte

Abstract

Nanographene oxide (nGO)-mediated hyperthermia has been increasingly investigated as a localized, minimally invasive anticancer therapeutic approach. Near InfraRed (NIR) light irradiation for inducing hyperthermia is particularly attractive, because biological systems mostly lack chromophores that absorb in this spectral window, facilitating the selective heating and destruction of cells which have internalized the NIR absorbing-nanomaterials. However, little is known about biological effects accompanying nGO-mediated hyperthermia at cellular and molecular levels. In this work, well-characterized pegylated nGO sheets with a hydrodynamic size of 300 nm were incubated with human Saos-2 osteosarcoma cells for 24 h and their internalization verified by flow cytometry and confocal microscopy. No effect on cell viability was observed after nGO uptake by Saos-2 cells. However, a proliferation delay was observed due to the presence of nGO sheets in the cytoplasm. 1H NMR metabolomics was employed to screen for changes in the metabolic profile of cells, as this could help to improve understanding of cellular responses to nanomaterials and provide new endpoint markers of effect. Cells internalizing nGO sheets showed noticeable changes in ...Continue Reading

Citations

Aug 8, 2020·Frontiers in Microbiology·Yuzheng HuangJun Cao
Feb 6, 2020·Frontiers in Microbiology·María Paulina RomeroNatalia Mayumi Inada
Dec 17, 2019·Colloids and Surfaces. B, Biointerfaces·Mónica CicuéndezIola F Duarte
Feb 14, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Mural QuadrosGunjan Verma

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