Metabolomics Analysis of the Toxic Effects of the Production of Lycopene and Its Precursors

Frontiers in Microbiology
April M MiguezMark P Styczynski

Abstract

Using cells as microbial factories enables highly specific production of chemicals with many advantages over chemical syntheses. A number of exciting new applications of this approach are in the area of precision metabolic engineering, which focuses on improving the specificity of target production. In recent work, we have used precision metabolic engineering to design lycopene-producing Escherichia coli for use as a low-cost diagnostic biosensor. To increase precursor availability and thus the rate of lycopene production, we heterologously expressed the mevalonate pathway. We found that simultaneous induction of these pathways increases lycopene production, but induction of the mevalonate pathway before induction of the lycopene pathway decreases both lycopene production and growth rate. Here, we aim to characterize the metabolic changes the cells may be undergoing during expression of either or both of these heterologous pathways. After establishing an improved method for quenching E. coli for metabolomics analysis, we used two-dimensional gas chromatography coupled to mass spectrometry (GCxGC-MS) to characterize the metabolomic profile of our lycopene-producing strains in growth conditions characteristic of our biosensor app...Continue Reading

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Citations

Dec 5, 2019·Nature Communications·Monica P McNerneyMark P Styczynski
Nov 19, 2019·Frontiers in Microbiology·Arshad RizviShekhar C Mande
Jul 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhaobao WangJianming Yang
Jun 12, 2021·Microbial Cell Factories·Simon J MoorePaul S Freemont
Jan 25, 2020·ACS Synthetic Biology·Monica P McNerneyMark P Styczynski
Feb 18, 2020·Industrial & Engineering Chemistry Research·April M MiguezMark P Styczynski
Sep 4, 2021·ACS Synthetic Biology·April M MiguezMark P Styczynski

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Datasets Mentioned

BETA
MTBLS642

Methods Mentioned

BETA
biosensors
biosensor
PCA

Software Mentioned

ChromaTOF
LOESS
MetaboAnalyst
MetPP

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