Metabolomics approach in lung tissue of septic rats and the interventional effects of Xuebijing injection using UHPLC-Q-Orbitrap-HRMS

Journal of Biochemistry
Tanye XuZhi Sun

Abstract

Sepsis is the dysregulated host response to an infection which leads to life-threatening organ dysfunction. Metabolomic profiling in bio-fluid or tissue is vital for elucidating the pathogenesis of sepsis and evaluating therapeutic effects of medication. In this study, an untargeted metabolomics approach was applied to study the metabolic changes in lung tissue of septic rats induced by cecal ligation and puncture (CLP) and investigate the treatment effects of Xubijing injection (XBJ). Metabolomics analyses were performed on ultra-high performance liquid chromatography-Q Exactive hybrid quadrupole-orbitrap high-resolution accurate mass spectrometry (UHPLC-Q-Orbitrap-HRMS) together with multivariate statistical analysis. A total of 26 differential metabolites between CLP and sham-operated group were identified. The altered metabolic pathways included energy metabolism, amino metabolism, lipid metabolism, fatty acid metabolism and hormone metabolism. Among the 26-varied metabolites, 15 were significantly regulated after XBJ treatment. The metabolic pathway network of sepsis was drawn to interpret the pathological feature of lung damage caused by sepsis and the underlying regulating mechanism of XBJ on the molecular levels. Our fi...Continue Reading

References

Jan 1, 1992·Physiological Reviews·R J Huxtable
Dec 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·W G MartinL Hill
Jan 1, 1996·Progress in Nucleic Acid Research and Molecular Biology·T W Traut, M E Jones
Dec 1, 2001·Science·T HlaM J Kluk
Apr 12, 2002·Current Medicinal Chemistry·Didier M LambertChristopher J Fowler
Jan 10, 2009·Nature Protocols·Daniel RittirschPeter A Ward
Apr 30, 2010·Experimental Lung Research·Junping SunJianxin Wang
Jun 12, 2010·Burns : Journal of the International Society for Burn Injuries·Xin-Ru LiuWei-Dong Zhang
Oct 5, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kathleen A StringerTheodore J Standiford
Oct 12, 2010·Analytical and Bioanalytical Chemistry·Steffen Neumann, Sebastian Böcker
Jun 16, 2011·Journal of Proteome Research·Richard P HorganLouise C Kenny
Oct 7, 2011·Intensive Care Medicine·José L Izquierdo-GarcíaJosé A Lorente
Apr 12, 2012·The Journal of Experimental Medicine·Ioanna NikitopoulouVassilis Aidinis
Apr 25, 2012·World Journal of Cardiovascular Diseases·Robert C BlockShaker A Mousa
Jan 9, 2013·Inhalation Toxicology·Jingjing ChenXiulan Zhao
Jul 17, 2013·Molecular BioSystems·Tao WenChoon Nam Ong
Aug 8, 2013·North American Journal of Medical Sciences·Ivan Surya PradiptaRizky Abdulah
Sep 1, 2007·Metabolomics : Official Journal of the Metabolomic Society·Lloyd W SumnerMark R Viant
Nov 8, 2015·European Journal of Pharmacology·Melody G Duvall, Bruce D Levy
Feb 24, 2016·JAMA : the Journal of the American Medical Association·Mervyn SingerDerek C Angus
Mar 12, 2016·Nature Reviews. Drug Discovery·David S Wishart
Sep 12, 2016·Analytical and Bioanalytical Chemistry·Zhicheng LiuGuowang Xu
Feb 25, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tengfei XuZhiqiang Liu
Mar 21, 2017·Journal of cardiology·Chao-Hung WangMing-Shi Shiao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antiparasitics

Antiparasitics are medications which are indicated for the treatment of parasitic diseases. Discover the latest research on antiparasitics here.