Metabolomics for high-resolution monitoring of the cellular physiological state in cell culture engineering

Metabolic Engineering
Panagiotis K ChrysanthopoulosMaria I Klapa

Abstract

Cell culture engineering has to-date used transcriptomic, proteomic, and metabolic flux analyses, attempting to resolve significant questions regarding cell culture performance. Despite the foreseen positive impact, the metabolomic analytical platform has not yet been vastly deployed. Presently, there is no published application of metabolomics to industrial-scale mammalian cell culture. We applied gas chromatography-mass spectrometry metabolomics to analyze baby hamster kidney (BHK) cells of various cell ages cultivated in high-cell density perfusion reactors at both laboratory and manufacturing scales. Cell growth, metabolic activity and protein productivity measurements, which are currently used to monitor the cellular physiological state, suggested consistency across bioreactors and over the course of the cultivation. Nevertheless, metabolomic profiling enabled the differentiation of cell cultures based on cell age, bioreactor scale and cell source. These results support the usefulness of metabolomics as a high resolution molecular analysis tool in cell culture engineering.

Citations

Mar 5, 2011·Journal of Biomolecular NMR·Nelly AranibarMichael D Reily
May 16, 2015·Biotechnology and Bioengineering·Huong LeChetan T Goudar
Jul 4, 2012·Metabolic Engineering·Neus Mora-BuyéAntoni Planas
Oct 12, 2010·Metabolic Engineering·Ryan P Nolan, Kyongbum Lee
Jun 11, 2015·Biotechnology and Bioengineering·Amanda M LewisZheng Jian Li
Jun 20, 2012·Biotechnology and Bioengineering·William Pooi Kat ChongYing Swan Ho
Mar 13, 2012·Biotechnology and Bioengineering·Stefanie DietmairLars K Nielsen
Oct 9, 2015·Analytical and Bioanalytical Chemistry·Blandine Madji HounoumSylvie Mavel
Jan 1, 2013·Computational and Structural Biotechnology Journal·Yuji Sawada, Masami Yokota Hirai
Jun 29, 2010·Journal of the American Chemical Society·Scott A BradleyAndreas Kaerner
Jan 17, 2012·Analytical Chemistry·Laetitia ShintuMarc-Emmanuel Dumas
Jun 30, 2018·Biotechnology Journal·Leila ZamaniVeronique Chotteau
Feb 26, 2013·Electrophoresis·Zacarías LeónAgustín Lahoz
Apr 19, 2019·Biotechnology and Bioengineering·Vania BertrandMassimo Morbidelli
Jun 12, 2019·Biotechnology and Bioengineering·Maja VodopivecTomaž Curk
Aug 17, 2019·Applied Microbiology and Biotechnology·Iman Shahidi Pour SaviziSeyed Abbas Shojaosadati
Mar 18, 2020·Critical Reviews in Biotechnology·Andrey S MarchevMilen I Georgiev
May 28, 2019·Metabolomics : Official Journal of the Metabolomic Society·Jordan WilkinsEugenia Trushina
Aug 26, 2017·Biotechnology Journal·Nicole J PiscopoKrishanu Saha
Nov 13, 2020·Analytical Chemistry·Arthur S EdisonSicong Zhang
Jun 25, 2021·Biotechnology Progress·Duc HoangSeongkyu Yoon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.