Metabolomics Integrated with Transcriptomics Reveals Redirection of the Phenylpropanoids Metabolic Flux in Ginkgo biloba

Journal of Agricultural and Food Chemistry
Jie MengGongke Zhou

Abstract

Ginkgo biloba is a monotypic species native to China with great economic and ecological values. Leaves extract of this tree contains about 24% flavonoids, which are widely used in the pharmaceutical industry. However, the flavonoids biosynthesis pathway is poorly understood in Ginkgo. In this study, we comprehensively compared the transcriptome and metabolite profiles of Ginkgo high-flavonoids mutant (ZY1) and Anlu1 (control) leaves. A total of 122 significantly changed metabolites and 1683 differentially expressed genes (DEGs), including 45 transcription factors, were identified in ZY1 compared to those in Anlu1. An integrated analysis of metabolic and transcriptomic data revealed that the abundances of some major flavonoids (especially flavone and flavonol) were most significantly increased, while other phenylpropanoid-derived products and lipids showed the most largely reduced abundances in ZY1 compared to those in Anlu1. Quantitative real-time polymerase chain reaction results confirmed the alterations in the expression levels of genes encoding components of pathways involved in phenylpropanoids and lipids. The redirection of metabolic flux may contribute to increased accumulation of flavonoid levels in ZY1 leaves. Our resu...Continue Reading

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Citations

Oct 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tao WangWenyan Zhang
Dec 18, 2020·Frontiers in Genetics·Ying GuoGuibin Wang
Jan 16, 2021·International Journal of Biological Macromolecules·Xiaoming YangFuliang Cao
Oct 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yaqiong WuLi-An Xu
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shipeng LiHuiyuan Ya
May 5, 2021·Journal of Agricultural and Food Chemistry·Fen WangYuanmao Jiang

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