Metabolomics of Mycobacterium tuberculosis

Methods in Molecular Biology
Madhumitha NandakumarKyu Y Rhee

Abstract

Enzymes fuel the biochemical activities of all cells. Their substrates and products thus offer a potential window into the physiologic state of a cell. Metabolomics focuses on the global, or systems-level, study of small molecules in a given biological system and thus provided an experimental tool with which to study cellular physiology on a global biochemical scale. While metabolomic studies of Mycobacterium tuberculosis are still in their infancy, recent studies have begun to deliver unique insights into the composition, organization, activity, and regulation of M. tuberculosis' physiologic network. Here, we outline practical methods for the culture, collection, and analysis of metabolomic samples from Mycobacterium tuberculosis that emphasize minimal sample perturbation, broad and native metabolite recovery, and sensitive, biologically agnostic metabolite detection.

Citations

Jul 7, 2015·Expert Review of Molecular Diagnostics·Emily R KoEphraim L Tsalik
Sep 28, 2016·ACS Infectious Diseases·Joanna C EvansValerie Mizrahi
Sep 11, 2019·Proceedings of the National Academy of Sciences of the United States of America·Zhe WangKyu Y Rhee
Jan 10, 2021·Nature Communications·Vitor MendesTom L Blundell
Apr 14, 2020·Tuberculosis·Michelle H LarsenRichard T Robinson
Jul 28, 2019·Microbiology Spectrum·Gabriel T MashabelaDigby F Warner
Aug 25, 2021·Proceedings of the National Academy of Sciences of the United States of America·Juhyeon LimHyungjin Eoh
Aug 26, 2021·Computational and Structural Biotechnology Journal·Isin T SakalliogluRobert Powers
Aug 29, 2021·Science Advances·Sarah M SchraderJulien Vaubourgeix

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Hindustan Antibiotics Bulletin
G Johansson
Progress in Industrial Microbiology
F D Sisler
International Journal of Geriatric Psychiatry
Lisa A PaigeDavid C Steffens
Expert Review of Molecular Diagnostics
Stephan BeiskenReza Salek
© 2021 Meta ULC. All rights reserved