Metabolomics of Neurotransmitters and Related Metabolites in Post-Mortem Tissue from the Dorsal and Ventral Striatum of Alcoholic Human Brain

Neurochemical Research
Mohammed Abul KashemVladimir J Balcar

Abstract

We report on changes in neurotransmitter metabolome and protein expression in the striatum of humans exposed to heavy long-term consumption of alcohol. Extracts from post mortem striatal tissue (dorsal striatum; DS comprising caudate nucleus; CN and putamen; P and ventral striatum; VS constituted by nucleus accumbens; NAc) were analysed by high performance liquid chromatography coupled with tandem mass spectrometry. Proteomics was studied in CN by two-dimensional gel electrophoresis followed by mass-spectrometry. Proteomics identified 25 unique molecules expressed differently by the alcohol-affected tissue. Two were dopamine-related proteins and one a GABA-synthesizing enzyme GAD65. Two proteins that are related to apoptosis and/or neuronal loss (BiD and amyloid-β A4 precursor protein-binding family B member 3) were increased. There were no differences in the levels of dopamine (DA), 3,4-dihydrophenylacetic acid (DOPAC), serotonin (5HT), homovanillic acid (HVA), 5-hydroxyindoleacetic acid (HIAA), histamine, L-glutamate (Glu), γ-aminobutyric acid (GABA), tyrosine (Tyr) and tryptophan (Tryp) between the DS (CN and P) and VS (NAc) in control brains. Choline (Ch) and acetylcholine (Ach) were higher and norepinephrine (NE) lower, in...Continue Reading

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Citations

Jul 28, 2016·Journal of Pharmaceutical and Biomedical Analysis·Carolina Gonzalez-RianoCoral Barbas
Feb 13, 2020·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Dingang ZhangYan Jiang
May 16, 2019·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Taija Voutilainen, Olli Kärkkäinen
Apr 4, 2017·Neural Plasticity·Malgorzata Jasinska, Elzbieta Pyza
Jul 26, 2018·Neurochemical Research·Mohammed A KashemVladimir J Balcar

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