Metabolomics of tracheal wash samples and exhaled breath condensates in healthy horses and horses affected by equine asthma

Journal of Breath Research
Marilena BazzanoFulvio Laus

Abstract

The present work characterized the metabolomic profile of tracheal wash (TW) and exhaled breath condensate (EBC) in healthy horses and horses with respiratory disease. Six asthma-affected horses (group A) and six healthy controls (group H) underwent clinical, endoscopic and cytologic examinations of upper airways to confirm the active phase of asthma. TW and EBC samples were collected from each animal and investigated by proton nuclear magnetic resonance (1H-NMR) metabolomic analysis. A total of ten out of 38 metabolites found in the TW were significantly different between the groups (p < 0.05). Higher concentrations of histamine and oxidant agents, such as glutamate, valine, leucine and isoleucine, as well as lower levels of ascorbate, methylamine, dimethylamine and O-phosphocholine, were found in group A compared to group H. Eight metabolites were found in equine EBC, namely methanol, ethanol, formate, trimethylamine, acetone, acetate, lactate and butanone, previously observed also in human EBC. Despite the fact that this was a pilot study, the results showed that the metabolomic analysis of TW and EBC has the potentiality to serve as a basis for diagnostic tools in horses with asthma.

References

Jul 21, 1977·The New England Journal of Medicine·M L SimenhoffR Doty
Jan 1, 1992·Journal of Cardiovascular Pharmacology·P VallanceS Moncada
Feb 1, 1980·Oral Surgery, Oral Medicine, and Oral Pathology·P A ThomasK M Kotian
Sep 1, 1981·Respiration Physiology·R M StewartD E Niewoehner
Aug 28, 1999·Lancet·F J KellyT Sandström
Feb 13, 2001·American Journal of Physiology. Endocrinology and Metabolism·D RajT Welbourne
May 16, 2001·Equine Veterinary Journal·R S PirieB C McGorum
May 16, 2003·The Veterinary Clinics of North America. Equine Practice·Renaud Léguillette
Oct 3, 2003·Veterinary Immunology and Immunopathology·Dorothy M AinsworthDouglas F Antczak
Jan 1, 2004·Veterinary Immunology and Immunopathology·Marie-Eve CordeauJean-Pierre Lavoie
Feb 26, 2004·American Journal of Respiratory and Critical Care Medicine·Aldo Baritussio
Oct 6, 2005·Experimental Lung Research·Christopher M DeatonFrank J Kelly
May 12, 2006·Redox Report : Communications in Free Radical Research·Christopher M Deaton
Nov 9, 2006·Metabolic Brain Disease·Alethéa G BarschakCarmen R Vargas
Apr 3, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kristine ChobanyanDimitrios Tsikas
Jun 10, 2008·Current Drug Targets·Nathalie Kirschvink, Petra Reinhold
Jun 10, 2008·Applied and Environmental Microbiology·Rina GonzálezElaine E Vaughan
Jul 26, 2008·The European Respiratory Journal·G de LaurentiisM Sofia
Sep 18, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Scott S BilleckeCrystal A Gadegbeku
Mar 5, 2009·NMR in Biomedicine·Ienaka TakedaCarolyn Slupsky
Sep 11, 2010·Applied Spectroscopy·Kristian Hovde LilandBjørn-Helge Mevik
Aug 10, 2011·Respirology : Official Journal of the Asian Pacific Society of Respirology·Mathilde LeclereJean-Pierre Lavoie
Dec 21, 2011·The European Respiratory Journal·Baljit K UbhiSusan C Connor
May 25, 2012·Journal of Breath Research·Agnes W BootsFrederik J van Schooten
Aug 3, 2012·Journal of Breath Research·Wolfram MiekischJochen K Schubert
Jan 31, 2013·Journal of Proteome Research·Guglielmo de LaurentiisAndrea Motta
Mar 23, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J JungG-S Hwang
Oct 29, 2013·Journal of Thoracic Disease·Annette G DentPaul V Zimmerman
Oct 30, 2013·Equine Veterinary Journal·R S Pirie
Nov 2, 2013·Veterinary Immunology and Immunopathology·E PadoanL Bargelloni
Sep 19, 2014·Journal of Veterinary Internal Medicine·A Niedzwiedz, Z Jaworski

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Citations

Aug 19, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Helena TranPanayiotis Loukopoulos
Sep 10, 2020·Frontiers in Veterinary Science·Laurent CouetilCyprianna Swiderski
Mar 12, 2021·Critical Reviews in Analytical Chemistry·Maryam KhoubnasabjafariAbolghasem Jouyban
May 1, 2021·Animals : an Open Access Journal From MDPI·Malwina SlowikowskaArtur Niedzwiedz

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