Metabolomics reveals differences of metal toxicity in cultures of Pseudomonas pseudoalcaligenes KF707 grown on different carbon sources

Frontiers in Microbiology
Sean C BoothRaymond J Turner

Abstract

Co-contamination of metals and organic pollutants is a global problem as metals interfere with the metabolism of complex organics by bacteria. Based on a prior observation that metal tolerance was altered by the sole carbon source being used for growth, we sought to understand how metal toxicity specifically affects bacteria using an organic pollutant as their sole carbon source. To this end metabolomics was used to compare cultures of Pseudomonas pseudoalcaligenes KF707 grown on either biphenyl (Bp) or succinate (Sc) as the sole carbon source in the presence of either aluminum (Al) or copper (Cu). Using multivariate statistical analysis it was found that the metals caused perturbations to more cellular processes in the cultures grown on Bp than those grown on Sc. Al induced many changes that were indicative of increased oxidative stress as metabolites involved in DNA damage and protection, the Krebs cycle and anti-oxidant production were altered. Cu also caused metabolic changes that were indicative of similar stress, as well as appearing to disrupt other key enzymes such as fumarase. Additionally, both metals caused the accumulation of Bp degradation intermediates indicating that they interfered with Bp metabolism. Together t...Continue Reading

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Citations

May 11, 2016·The Science of the Total Environment·Catarina R Marques
Oct 4, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Małgorzata PatejkoMichał J Markuszewski
Jul 13, 2018·Metallomics : Integrated Biometal Science·Qixiao ZhaiWei Chen
Jul 20, 2018·International Journal of Systematic and Evolutionary Microbiology·Stefano FediDavide Zannoni
Sep 11, 2018·Critical Reviews in Biotechnology·Arun Kumar DangiPratyoosh Shukla
Feb 3, 2020·Ecotoxicology and Environmental Safety·Jong Cheol ShonJong-Su Seo
Sep 3, 2021·Critical Reviews in Microbiology·Ayon PalParimal Mandal

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Methods Mentioned

BETA
PCA

Software Mentioned

IDEA
R
- Project for Statistical Computing
Automated Mass Spectral Deconvolution and Identification Softw...
BLAST
R script
mBROLE
MET
SIMCA P +

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