Metabonomic analysis of ovarian tumour cyst fluid by proton nuclear magnetic resonance spectroscopy

Oncotarget
Michael KyriakidesMona El-Bahrawy

Abstract

The majority of ovarian tumours are of the epithelial type, which can be sub classified as benign, borderline or malignant. Epithelial tumours usually have cystic spaces filled with cyst fluid, the metabolic profile of which reflects the metabolic activity of the tumour cells, due to their close proximity. The approach of metabonomics using 1H-NMR spectroscopy was employed to characterize the metabolic profiles of ovarian cyst fluid samples (n = 23) from benign, borderline and malignant ovarian tumours in order to shed more light into ovarian tumour and cancer development. The analysis revealed that citrate was elevated in benign versus malignant tumours, while the amino acid lysine was elevated in malignant versus non-malignant tumours, both at a 5% significance level. Choline and lactate also had progressively increasing levels from benign to borderline to malignant samples. Finally, hypoxanthine was detected exclusively in a sub-cohort of the malignant tumours. This metabonomic study demonstrates that ovarian cyst fluid samples have potential to be used to distinguish between the different types of ovarian epithelial tumours. Furthermore, the respective metabolic profiles contain mechanistic information which could help iden...Continue Reading

References

Jan 1, 1989·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·C Des RosiersE Gerlach
Aug 1, 1996·The American Journal of Medicine·C J LinkE C Kohn
Nov 7, 2000·Cancer·B A GoffC H Melancon
Nov 20, 2002·Chemical Research in Toxicology·Hector C KeunJeremy K Nicholson
Feb 24, 1956·Science·O WARBURG
Nov 2, 2004·Nature Reviews. Cancer·Robert A Gatenby, Robert J Gillies
Oct 18, 2005·Cancer Cell·Georgia HatzivassiliouCraig B Thompson
Oct 19, 2005·Cancer Research·Egidio IorioFranca Podo
Oct 22, 2005·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A du BoisUNKNOWN Organizational team of the two prior International OCCC
Jan 24, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ann F Chambers, Barbara C Vanderhyden
Oct 5, 2006·Critical Reviews in Oncology/hematology·Nicoletta ColomboIgnace Vergote
Feb 22, 2008·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Nov 29, 2008·The Lancet Oncology·Louis Dubeau
Jan 22, 2009·Cancer·Donald M LamkinSusan K Lutgendorf
Jul 14, 2009·NMR in Biomedicine·Eva KolwijckRon A Wevers
Aug 8, 2009·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Eva KolwijckLeon F A G Massuger
Apr 7, 2011·Investigative Radiology·Anastassia EsseridouFrancesco Sardanelli
Nov 21, 2012·Journal of Proteome Research·Tao ZhangKang Li

❮ Previous
Next ❯

Citations

Sep 25, 2017·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Jun Hwa LeeSeung Cheol Kim
Apr 1, 2016·Metabolomics : Official Journal of the Metabolomic Society·Onur TurkogluRay Bahado-Singh
Apr 20, 2018·NMR in Biomedicine·Guro F GiskeødegårdTone F Bathen
Dec 19, 2019·Metabolites·Elin StrandCamilla Krakstad
Oct 23, 2020·Metabolites·Asia SaorinGiuseppe Corona
Oct 28, 2020·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Yousra Ahmed-SalimSrdjan Saso
Jan 21, 2017·Analytical Chemistry·G A Nagana Gowda, Daniel Raftery

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
xenograft
NMR
PCA

Software Mentioned

Prism
TopSpin
MATLAB
GraphPad
SIMCA

Related Concepts

Related Feeds

Adenocarcinoma, Clear Cell

Clear Cell Adenocarcinoma is a tumor that arises in the female genital tract and is characterized by cells that appear clear under the microscope. Discover the latest research here.

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Cancer -Omics

A variety of different high-throughput technologies can be used to identify the complete catalog of changes that characterize the molecular profile of cohorts of tumor samples. Discover the latest insights gained from cancer 'omics' in this feed.