Metabonomics study of the effects of traditional Chinese medicine formula Ermiaowan on hyperuricemic rats

Journal of Separation Science
Zhongbao WeiXiaobo Qu

Abstract

To explore the global mechanism of Ermiaowan on hyperuricemia regulation, the holistic function of Ermiaowan for hyperuricemia in rats was firstly assessed by the urinary metabonomics method which was based on ultra-high performance liquid chromatography with electrospray ionization quadrupole time-of-flight mass spectrometry. The urinary targeted metabonomics approach combined with the serum biochemical analysis and histological assay was conducted to verify the research result. As a result, the significant differences in metabolic profiles were observed from Ermiaowan-treated group, model group, and healthy control group by using multivariate statistical approaches. Twenty therapeutic related metabolites were identified in response to the therapeutic effects of Ermiaowan, which were mainly associated with purine metabolism, pyrimidine metabolism, tryptophan metabolism, tricarboxylic acid cycle, and tyrosine metabolism. In addition, the urinary targeted metabonomics study showed that Ermiaowan can better restore the disturbed pathways than Phellodendri cortex and Atractylodis rhizome. The biochemical assay and histopathological assay confirmed that Ermiaowan could significantly reduce uric acid and fibrosis areas of kidney. Th...Continue Reading

References

Jan 1, 1985·American Journal of Epidemiology·F N BrandW P Castelli
Nov 30, 1993·Clinica Chimica Acta; International Journal of Clinical Chemistry·I P KemaF A Muskiet
Nov 10, 2000·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·T MiyazakiT Niwa
Oct 10, 2002·American Journal of Nephrology·Takahiko NakagawaRichard J Johnson
Sep 6, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Renata CaudarellaSegio Stefoni
Apr 21, 2005·Kidney International·Uday M KhoslaRichard J Johnson
Dec 27, 2005·Current Pharmaceutical Design·G K GlantzounisD A Galaris
Jan 22, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Jörg C SchefoldPetra Reinke

❮ Previous
Next ❯

Citations

Nov 10, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Peng WuWeifeng Sun
Apr 29, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Bixia HuangJun Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antiparasitics

Antiparasitics are medications which are indicated for the treatment of parasitic diseases. Discover the latest research on antiparasitics here.