Metagenomic analyses of the gut microbiota associated with colorectal adenoma

PloS One
Keisuke SaitoToshifumi Ohkusa

Abstract

Recent studies have suggested an association between certain members of the Fusobacterium genus, especially F. nucleatum, and the progression of advanced colorectal carcinoma (CRC). We assessed such an association of the gut microbiota in Japanese patients with colorectal adenoma (CRA) or intramucosal CRC using colonoscopy aspirates. We analyzed samples from 81 Japanese patients, including 47 CRA and 24 intramucosal CRC patients, and 10 healthy subjects. Metagenomic analysis of the V3-V4 region of the 16S ribosomal RNA gene was performed. The linear discriminant analysis (LDA) effect size (LEfSe) method was used to examine microbial dysbiosis, revealing significant differences in bacterial abundances between the healthy controls and CRA or intramucosal CRC patients. In particular, F. varium was statistically more abundant in patients with CRA and intramucosal CRC than in healthy subjects. Here, we present the metagenomic profile of CRA and intramucosal CRC and demonstrate that F. varium is at least partially involved in the pathogenesis of CRA and intramucosal CRC.

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Citations

Jan 26, 2021·Oxidative Medicine and Cellular Longevity·Sujuan DingGang Liu
Feb 13, 2021·NAR Genomics and Bioinformatics·Maria Muñoz-BenaventThilo Muth
Feb 27, 2021·Enfermedades infecciosas y microbiología clínica·Paola Villar-OrtegaJosé Gutiérrez-Fernández
Jun 3, 2021·International Journal of Molecular Sciences·Shuhei FukunagaTakuji Torimura
Jul 3, 2021·Cancers·Francesca AprileStefano Pontone

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Methods Mentioned

BETA
PCR

Software Mentioned

MiSeq
BLASTN
QIIME
UCHIME
StatView
LEfSe
Quantitative Insights Into Microbial Ecology ( QIIME )

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