Metagenomic analysis and functional characterization of the biogas microbiome using high throughput shotgun sequencing and a novel binning strategy

Biotechnology for Biofuels
Stefano CampanaroIrini Angelidaki

Abstract

Biogas production is an economically attractive technology that has gained momentum worldwide over the past years. Biogas is produced by a biologically mediated process, widely known as "anaerobic digestion." This process is performed by a specialized and complex microbial community, in which different members have distinct roles in the establishment of a collective organization. Deciphering the complex microbial community engaged in this process is interesting both for unraveling the network of bacterial interactions and for applicability potential to the derived knowledge. In this study, we dissect the bioma involved in anaerobic digestion by means of high throughput Illumina sequencing (~51 gigabases of sequence data), disclosing nearly one million genes and extracting 106 microbial genomes by a novel strategy combining two binning processes. Microbial phylogeny and putative taxonomy performed using >400 proteins revealed that the biogas community is a trove of new species. A new approach based on functional properties as per network representation was developed to assign roles to the microbial species. The organization of the anaerobic digestion microbiome is resembled by a funnel concept, in which the microbial consortium ...Continue Reading

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Citations

Jan 31, 2017·The Science of the Total Environment·Stefano CampanaroViviana Corich
Dec 13, 2016·Applied Microbiology and Biotechnology·Xinyu ZhuIrini Angelidaki
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Datasets Mentioned

BETA
PRJNA283298
SRP058179

Methods Mentioned

BETA
phosphotransferase

Software Mentioned

RAST metagenomics analysis server
GroopM
RPSBLAST
Trimmomatic
MeV
MG
KOBAS
R package
BLASTN
genomecov

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