Metagenomic analysis reveals adaptations to a cold-adapted lifestyle in a low-temperature acid mine drainage stream

FEMS Microbiology Ecology
Maria LiljeqvistMark Dopson

Abstract

An acid mine drainage (pH 2.5-2.7) stream biofilm situated 250 m below ground in the low-temperature (6-10°C) Kristineberg mine, northern Sweden, contained a microbial community equipped for growth at low temperature and acidic pH. Metagenomic sequencing of the biofilm and planktonic fractions identified the most abundant microorganism to be similar to the psychrotolerant acidophile, Acidithiobacillus ferrivorans. In addition, metagenome contigs were most similar to other Acidithiobacillus species, an Acidobacteria-like species, and a Gallionellaceae-like species. Analyses of the metagenomes indicated functional characteristics previously characterized as related to growth at low temperature including cold-shock proteins, several pathways for the production of compatible solutes and an anti-freeze protein. In addition, genes were predicted to encode functions related to pH homeostasis and metal resistance related to growth in the acidic metal-containing mine water. Metagenome analyses identified microorganisms capable of nitrogen fixation and exhibiting a primarily autotrophic lifestyle driven by the oxidation of the ferrous iron and inorganic sulfur compounds contained in the sulfidic mine waters. The study identified a low di...Continue Reading

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K Maeda, Y Imae
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J GoldsteinM Inouye
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Dec 24, 1997·Applied and Environmental Microbiology·D Emerson, C Moyer
Jan 5, 1999·Applied and Environmental Microbiology·M Dopson, E B Lindstrom
Aug 10, 2002·Bioinformatics·Peter D KarpPedro Romero
Aug 13, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Michael H W Weber, Mohamed A Marahiel
Jun 17, 2003·International Journal of Systematic and Evolutionary Microbiology·Kevin T FinneranDerek R Lovley
Oct 8, 2003·Systematic Biology·Stéphane Guindon, Olivier Gascuel
Nov 7, 2003·Nucleic Acids Research·Clotilde Claudel-RenardDaniel Kahn
Jan 2, 2004·Microbiology·Jack A GilbertJohanna Laybourn-Parry
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Jun 14, 2005·Biochimica Et Biophysica Acta·Moeava Tehei, Giuseppe Zaccai
Jul 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Barbara A MethéClaire M Fraser
Feb 8, 2006·Applied and Environmental Microbiology·Joseph J GrzymskiAlison E Murray
Mar 1, 1997·Applied and Environmental Microbiology·L O MartinsH Santos
Jun 23, 2006·Nucleic Acids Research·George W Owttrim
Jul 6, 2006·Applied and Environmental Microbiology·T Z DeSantisG L Andersen
Dec 21, 2006·BMC Evolutionary Biology·Nelson AvonceGabriel Iturriaga
Dec 26, 2006·Biotechnology and Bioengineering·Mark DopsonJaakko A Puhakka
Feb 17, 2007·Biotechnology and Bioengineering·Daniel KupkaOlli H Tuovinen
Aug 9, 2008·The Biochemical Journal·Julie G Donaldson
Oct 23, 2008·Nucleic Acids Research·William KlimkeTatiana Tatusova
Nov 14, 2008·Nucleic Acids Research·J R ColeJ M Tiedje
May 2, 2009·International Journal of Systematic and Evolutionary Microbiology·D Barrie JohnsonKevin B Hallberg
Aug 25, 2009·Genome Biology·Gregory J DickJillian F Banfield

❮ Previous
Next ❯

Citations

Jan 23, 2016·FEMS Microbiology Reviews·Jérémy Andres, Philippe N Bertin
Apr 7, 2016·Trends in Microbiology·Li-Nan HuangWen-Sheng Shu
Jun 12, 2016·Research in Microbiology·Harold NuñezRaquel Quatrini
Mar 14, 2018·Life·Paula M Tribelli, Nancy I López
Dec 9, 2017·Genome Announcements·Andrey V MardanovNikolai V Ravin
Jul 17, 2019·Journal of Applied Microbiology·A M SayedM E Rateb
Aug 12, 2014·Applied Microbiology and Biotechnology·Mark Dopson, David S Holmes
Jan 11, 2017·Canadian Journal of Microbiology·Ryan R AuldThomas J S Merritt
Aug 14, 2020·FEMS Microbiology Ecology·Vaso TaleskiSlaviša Stanković
Feb 25, 2017·Frontiers in Plant Science·Kamlesh K MeenaParamjit S Minhas
Jul 18, 2018·Frontiers in Microbiology·Oscar E AguinagaJon K Pittman
Sep 22, 2019·Journal of Hazardous Materials·Patricia GiovanellaLara D Sette
Jul 12, 2020·Environment International·Tangran HuoSitong Liu
Apr 28, 2021·Environmental Microbiome·Peter OsborneWilfried Haerty

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.