Metagenomic insights into the microbial diversity in manganese-contaminated mine tailings and their role in biogeochemical cycling of manganese

Scientific Reports
Shreya Ghosh, Alok Prasad Das

Abstract

To extend the knowledge on the microbial diversity of manganese rich environments, we performed a clone library based study using metagenomic approach. Pyrosequencing based analysis of 16S rRNA genes were carried out on an Illumina platform to gain insights into the bacterial community inhabiting in a manganese mining site and the taxonomic profiles were correlated with the inherent capacities of these strains to solubilise manganese. The application of shot gun sequencing in this study yielded results which revealed the highest prevalence of Proteobacteria (42.47%), followed by Actinobacteria (23.99%) in the area of study. Cluster of orthologous group (COG) functional category has 85,066 predicted functions. Out of which 11% are involved in metabolism of amino acid, 9% are involved in production and conversion of energy while Keto Encyclopedia of Gene and Genomes (KEGG) functional category has 107,388 predicted functions, out of which 55% are involved in cellular metabolism, 15% are environmental and information processing and 12% are genetic information processing in nature. The isolated microbial consortia demonstrated visible growth in presence of high concentrations of Mn. Solubilisation studies resulted in 86% of manganes...Continue Reading

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Citations

Jun 13, 2019·Omics : a Journal of Integrative Biology·Ilaria LaudadioClaudia Carissimi
Jun 26, 2020·Frontiers in Bioengineering and Biotechnology·Javad Aminian-DehkordiIvan Mijakovic
Oct 5, 2020·Environmental Science and Pollution Research International·Shreya GhoshAlok Prasad Das
Nov 21, 2021·Applied Biochemistry and Biotechnology·Joyasree BhattacharjeeAlok Prasad Das

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Datasets Mentioned

BETA
PRJNA360904

Methods Mentioned

BETA
environmental stresses
X-ray
PCR

Software Mentioned

perl
BlastN
MEGAN
CLC Genomics Workbench
Prodigal
PFAM
FIGfams
Covaris
COGNIZER

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