Abstract
In recent years, there have been particular emphases worldwide on the development and optimization of bioprocesses for the utilization of biomass. An essential component of the biomass processing conduit has been the need for robust biocatalysts as high-performance tools for both the depolymerization of lignocellulosic biomass and synthesis of new high-value bio-based chemical entities. Through functional screening of the metagenome of the hindgut bacterial symbionts of a termite, Trinervitermes trinervoides, we discovered open reading frames for 25 cellulases and hemicellulases. These were classified into 14 different glycoside hydrolase (GH) families: eight GH family 5; four GH9, two GH13, and one each in GH2, GH10, GH11, GH26, GH29, GH43, GH44, GH45, GH67, and GH94 families. Of these, eight were overexpressed and partially characterized to be shown to be endocellulases (GH5C, GH5E, GH5F, and GH5G), an exocellulase (GH5D), endoxylanases (GH5H and GH11), and an α-fucosidase (GH29). The GH11 (Xyl1) was of particular interest as it was discovered to be a multimodular β-1,4-xylanase, consisting of a catalytic domain and two carbohydrate-binding modules (CBMs). The CBM functions to selectively bind insoluble xylan and increases th...Continue Reading
References
Jul 25, 1991·Nucleic Acids Research·R H DonJ S Mattick
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Aug 15, 1970·Nature·U K Laemmli
Apr 1, 1982·Applied and Environmental Microbiology·R M Teather, P J Wood
Dec 1, 1994·Current Opinion in Structural Biology·J D McCarter, S G Withers
Feb 1, 1993·Bioscience, Biotechnology, and Biochemistry·K SakkaK Shimada
Jun 1, 1994·Trends in Biochemical Sciences·R D AppelD F Hochstrasser
Jun 1, 1996·The Biochemical Journal·B Henrissat, A Bairoch
Oct 18, 1996·Journal of Molecular Biology·U Krengel, B W Dijkstra
Dec 5, 1998·Biotechnology Progress·Y ZhengG T Tsao
Feb 4, 1999·Protein Engineering·S FushinobuH Matsuzawa
Jun 15, 1999·Journal of Bacteriology·S ShulamiY Shoham
Jun 22, 2000·Journal of Molecular Biology·M D JoshiL P McIntosh
Mar 29, 2001·Bioscience, Biotechnology, and Biochemistry·M K AliK Ohmiya
Mar 26, 2002·Journal of Biotechnology·Amalia SapagEric Depiereux
Dec 17, 2002·Current Opinion in Biotechnology·Patrick LorenzJürgen Eck
Aug 2, 2003·Biotechnology Progress·Anders WingrenGuido Zacchi
Jun 30, 2004·Journal of Molecular Biology·Jannick Dyrløv BendtsenSøren Brunak
Jul 17, 2004·Microbiology·Helmuth AdelsbergerWolfgang H Schwarz
Dec 27, 2005·The Journal of Physical Chemistry. B·Jennifer L DashnauJane M Vanderkooi
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Oded ShoseyovIlan Levy
Nov 23, 2007·Nature·Falk WarneckeJared R Leadbetter
Jun 22, 2010·Journal of Bacteriology·Yejun HanIsaac K O Cann
Feb 12, 2011·Biotechnology for Biofuels·Valdeir Arantes, Jack N Saddler
May 7, 2011·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Aug 6, 2011·Biotechnology for Biofuels·Luen-Luen LiDaniel van der Lelie
Nov 10, 2011·Biotechnology Advances·Gabriel PaësJohnny Beaugrand
May 1, 2012·Molecular Biotechnology·Warin DeesukonWasana Sukhumsirichart
Oct 23, 2012·Current Microbiology·K J RashamuseD Brady
Feb 1, 2013·Biotechnology for Biofuels·Junhua ZhangLiisa Viikari
Apr 30, 2013·Biotechnology Advances·Jinfeng Ni, Gaku Tokuda
May 8, 2013·Applied Microbiology and Biotechnology·Konanani RashamuseDean Brady
May 16, 2013·Biotechnology for Biofuels·Géraldine BastienMichael O'Donohue
Dec 18, 2013·Applied Microbiology and Biotechnology·Sunil Subhash GhatgeSeon-Won Kim
Feb 4, 2014·Nature Reviews. Microbiology·Andreas Brune
Aug 1, 2014·Applied Microbiology and Biotechnology·Rosana GoldbeckFabio M Squina
Oct 24, 2014·Annual Review of Entomology·Michael E Scharf
Jul 1, 2015·Current Opinion in Chemical Biology·Timothy D H Bugg, Rahman Rahmanpour
Aug 28, 2015·Biochemistry and Biophysics Reports·Nobalanda MokoenaKonanani Rashamuse
Citations
May 18, 2018·Chemical Communications : Chem Comm·Roger A Sheldon, Dean Brady
Nov 30, 2018·PLoS Computational Biology·Ryman ShokoHugh Patterton
Dec 7, 2018·Proceedings of the National Academy of Sciences of the United States of America·Gaku TokudaAndreas Brune
Oct 14, 2017·FEMS Microbiology Letters·Francesca BeriniFlavia Marinelli
Aug 18, 2018·The ISME Journal·Ning LiuQian Wang
Apr 3, 2019·ChemSusChem·Roger A Sheldon, Dean Brady
Feb 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mpho S MafaBrett I Pletschke
Oct 13, 2020·Frontiers in Microbiology·Digvijay Verma, Tulasi Satyanarayana
Mar 27, 2021·Science·Doron LevinEran Segal
Oct 5, 2020·International Journal of Biological Macromolecules·Raul Alcântara Teixeira LimaRicardo Henrique Krüger