Metagenomic Profiling of Soil Microbes to Mine Salt Stress Tolerance Genes

Frontiers in Microbiology
Vasim AhmedNar S Chauhan

Abstract

Osmotolerance is one of the critical factors for successful survival and colonization of microbes in saline environments. Nonetheless, information about these osmotolerance mechanisms is still inadequate. Exploration of the saline soil microbiome for its community structure and novel genetic elements is likely to provide information on the mechanisms involved in osmoadaptation. The present study explores the saline soil microbiome for its native structure and novel genetic elements involved in osmoadaptation. 16S rRNA gene sequence analysis has indicated the dominance of halophilic/halotolerant phylotypes affiliated toProteobacteria, Actinobacteria, Gemmatimonadetes, Bacteroidetes, Firmicutes, andAcidobacteria. A functional metagenomics approach led to the identification of osmotolerant clones SSR1, SSR4, SSR6, SSR2 harboringBCAA_ABCtp, GSDH, STK_Pknb, andduf3445genes. Furthermore, transposon mutagenesis, genetic, physiological and functional studies in close association has confirmed the role of these genes in osmotolerance. Enhancement in host osmotolerance possibly though the cytosolic accumulation of amino acids, reducing equivalents and osmolytes involvingBCAA-ABCtp, GSDH, andSTKc_PknB. Decoding of the genetic elements pre...Continue Reading

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Citations

Nov 5, 2019·Journal of Basic Microbiology·Gautam AnandShilpi Sharma
Sep 30, 2020·Critical Reviews in Microbiology·Satish KumarYogesh Shouche
Dec 11, 2019·Extremophiles : Life Under Extreme Conditions·Dai-Di ChenWen-Jun Li
Oct 8, 2020·Journal, Genetic Engineering & Biotechnology·Sonika GuptaAnuradha Sourirajan
Jan 4, 2022·Environmental Monitoring and Assessment·Maria Grazia BonomoGiovanni Salzano

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Datasets Mentioned

BETA
SRS2727172

Methods Mentioned

BETA
transgenic

Software Mentioned

Quantitative Insights Into Microbial Ecology ( QIIME )
BLASTP
QIIME
Lasergene
NsitePred
Basic Local Alignment and Search Tool ( BLAST )
uclust
Man
MEGA7
Seq

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