Metagenomics analysis of red blood cell and fresh-frozen plasma units

Transfusion
Pierre LauOlivier Preynat-Seauve

Abstract

Although the risk of transmitting infectious agents by blood transfusion is dramatically reduced after donor selection, leukoreduction, and laboratory testing, some could still be present in donor's blood. A description of metagenomes in blood products eligible for transfusion represents relevant information to evaluate the risk of pathogen transmission by transfusion. Detection of viruses, bacteria, and fungi genomes was made by high-throughput sequencing (HTS) of 600 manufactured blood products eligible for transfusion: 300 red blood cell (RBC) and 300 fresh-frozen plasma (FFP) units. Anelloviruses and human pegivirus, frequent in the blood of healthy individuals, were found. Human papillomavirus type 27 and Merkel cell polyomavirus, present on the skin, were also detected. Unexpectedly, astrovirus MLB2 was identified and characterized in a FFP unit. The presence of astrovirus MLB2 was confirmed in donor's blood and corresponded to an asymptomatic acute viremia. Sequences of bacteria and fungi were also detected; they are likely the result of environmental contamination. This study demonstrates that HTS is a promising tool for detecting common and less frequent infectious pathogens in blood products.

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Citations

Apr 18, 2018·Transfusion·Sophie Waldvogel AbramowskiOlivier Preynat-Seauve
Jul 20, 2018·Vox Sanguinis·Francisco BritoOlivier Preynat-Seauve
Dec 18, 2019·Transfusion Medicine·Lei GaoChengyao Li
Oct 22, 2019·Transfusion·Sergio KammingaHans Zaaijer
May 1, 2021·Emerging Microbes & Infections·Samuel CordeyLaurent Kaiser
Aug 8, 2021·Microorganisms·Stefan PanaiotovReni Kalfin
Jun 14, 2019·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Sophie Waldvogel-AbramowskiOlivier Preynat-Seauve

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