Metagenomics for taxonomy profiling: tools and approaches.

Bioengineered
Mukesh Kumar AwasthiZengqiang Zhang

Abstract

The study of metagenomics is an emerging field that identifies the total genetic materials in an organism along with the set of all genetic materials like deoxyribonucleic acid and ribose nucleic acid, which play a key role with the maintenance of cellular functions. The best part of this technology is that it gives more flexibility to environmental microbiologists to instantly pioneer the immense genetic variability of microbial communities. However, it is intensively complex to identify the suitable sequencing measures of any specific gene that can exclusively indicate the involvement of microbial metagenomes and be able to advance valuable results about these communities. This review provides an overview of the metagenomic advancement that has been advantageous for aggregation of more knowledge about specific genes, microbial communities and its metabolic pathways. More specific drawbacks of metagenomes technology mainly depend on sequence-based analysis. Therefore, this 'targeted based metagenomics' approach will give comprehensive knowledge about the ecological, evolutionary and functional sequence of significantly important genes that naturally exist in living beings either human, animal and microorganisms from distinctive ...Continue Reading

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Citations

Dec 15, 2020·Journal of Hazardous Materials·Sanjeev Kumar AwasthiMohammad J Taherzadeh
Jan 14, 2021·Bioresource Technology·Shashi Kant BhatiaGopalakrishnan Kumar
Mar 2, 2021·Frontiers in Bioengineering and Biotechnology·Sandhya MishraShaohua Chen
Mar 13, 2021·Frontiers in Bioengineering and Biotechnology·Bashar Amer, Edward E K Baidoo
Dec 11, 2020·Bioresource Technology·Shiyi QinMohammad J Taherzadeh
Jun 10, 2021·Applied and Environmental Microbiology·O G G Almeida, E C P De Martinis

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Methods Mentioned

BETA
PCR
Illumina sequencing
electrophoresis
Pool-seq

Software Mentioned

MetaPhlAn2
Genovo
Storm
CosmosID
GPA
Proteo
BLASTP
Trimmomatic
Illumina
MegaBLAST

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