Metal accumulation and expression of genes encoding for metallothionein and copper transporters in a chronically exposed wild population of the fish Hyphessobrycon luetkenii

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
Sandra Isabel Moreno AbrilAdalto Bianchini

Abstract

In the present study, metal (As, Cd, Cu, Fe, Mn, Pb and Zn) accumulation and expression of genes involved in metal metabolism (metallothioneins, ATP7A and CTR1) were evaluated in gills and muscle of the fish Hyphessobrycon luetkenii living in the João Dias creek, a site historically (~1870-1996) contaminated with a metal mixture associated with copper mining (Minas do Camaquã, southern Brazil). Fish were collected in a metal impacted site of the João Dias creek and kept in a cage at this site (PP fish) or translocated to a non-metal impacted reach of this creek (PC fish). Gill metal concentrations and metallothionein gene expression were lower in PC fish than in PP fish at any experimental time (24, 48 and 72 h). In muscle, no significant changes were observed. These findings indicate that metal accumulated in gills of wild fish chronically exposed to the metal mixture are more easily excreted than those accumulated in the muscle. In this case, expression of gene encoding for metallothionein is shown to play a key role in the regulation of metal accumulation in gills of H. luetkenii living in an area historically contaminated with a metal mixture associated with copper mining.

References

Jun 19, 2001·Anais Da Academia Brasileira De Ciências·E D BidoneJ L Maddock
Jun 1, 2005·Ecotoxicology and Environmental Safety·Gregory G PylePatrice Couture
Jun 21, 2006·Biochimica Et Biophysica Acta·Kuppusamy Balamurugan, Walter Schaffner
May 29, 2007·Archives of Biochemistry and Biophysics·Sharon La Fontaine, Julian F B Mercer
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Jun 29, 2010·The Journal of Heredity·Andrew WhiteheadMarjorie F Oleksiak
Apr 7, 2012·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Marleen EyckmansGudrun De Boeck
Jun 19, 2012·Nature Reviews. Genetics·Irene Gallego RomeroYoav Gilad
Jun 20, 2013·Genome Biology and Evolution·Maria Warnefors, Henrik Kaessmann
Mar 19, 2014·Frontiers in Pharmacology·Lu ZhaoFudi Wang
Jan 6, 2015·Environmental Toxicology and Chemistry·Robert C Santore, Adam C Ryan
Feb 25, 2017·Environmental Science and Pollution Research International·Yuyu JiaZhaoguang Yang

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Citations

Nov 30, 2019·Current Pharmaceutical Biotechnology·Qingyun JiaLan Wang

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