PMID: 8599605Feb 29, 1996Paper

Metal binding and ferritin immunoreactivity in a high molecular weight fraction from rat brain

Biochimica Et Biophysica Acta
S San-Marina, D M Nicholls

Abstract

A high molecular weight protein fraction (PI) from rat brain that exhibited heat- and acid-resistant properties like ferritin was characterized with respect to its reactivity with spleen ferritin antiserum, iron (Fe) content and Fe and aluminum (Al) binding. This fraction eluted on Sepharose 4B and Sepharose 6B columns with an apparent Mr of 2 100 000. A second Fe-rich fraction (PII) with an Mr of 500 000 showed lower anti-ferritin activity. The PI and PII fractions from liver, when prepared without the heat and acid steps, were similar to those found in brain. In both tissues, the PI fraction had a low Fe/ferritin ratio (0.28 +/- 0.08 microgram/microgram for brain and 0.22 +/- 0.05 microgram/microgram for liver). Similarly the PII fractions had high Fe/ferritin ratios (1.48 +/- 0.44 microgram/microgram for brain and 1.34 +/- 0.10 microgram/microgram for liver). Analysis of Fe binding to brain PI revealed one high affinity site (Kd = 157 +/- 30.0 X 10(-6) M; Bmax = 1.42 +/- 0.11 micromol Fe/mg protein) and one low affinity site (Kd = 890 +/- 100 X 10(-6) M; Bmax = 5.24 +/- 0.96 micromol Fe/mg protein). Removal of Fe from brain PI with 1% thioglycollic acid solution prior to Fe binding, increased binding affinity at both sites, ...Continue Reading

References

Nov 18, 1975·Biochimica Et Biophysica Acta·L VulimiriH N Munro
Oct 15, 1992·The Biochemical Journal·D J DedmanP M Harrison
Sep 1, 1991·Neurobiology of Aging·J T Fleming, J G Joshi
Jan 1, 1990·The International Journal of Biochemistry·D M NichollsA C Miller
Jan 1, 1990·Methods in Enzymology·T Pohl
Jan 1, 1989·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·B G AtkinsonT W Blaker
Feb 1, 1989·Journal of Neurochemistry·P RiedererM B Youdim
Nov 1, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·M Cochran, V Chawtur
Apr 1, 1988·Brain Research·N A MumaD L Price
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J Fleming, J G Joshi
Nov 1, 1968·The Biochemical Journal·M A Williams, P M Harrison
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D Price, J G Joshi
Apr 1, 1995·The International Journal of Biochemistry & Cell Biology·D M NichollsA C Miller
Aug 1, 1994·Journal of Neurochemistry·A J Roskams, J R Connor
Dec 1, 1993·The Journal of Comparative Neurology·S A Benkovic, J R Connor

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Citations

Dec 14, 2004·Journal of Industrial Microbiology & Biotechnology·Fang LiuMasaaki Yasuda
Jul 9, 2005·Journal of Biomaterials Science. Polymer Edition·Handan YavuzAdil Denizli
May 16, 2002·Journal of Inorganic Biochemistry·Michael J Hynes, Máirtín O Coinceanainn

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