Metal binding specificity of the MntABC permease of Neisseria gonorrhoeae and its influence on bacterial growth and interaction with cervical epithelial cells.

Infection and Immunity
Karen H L LimA G McEwan

Abstract

mntABC from Neisseria gonorrhoeae encodes an ABC permease which includes a periplasmic divalent cation binding receptor protein of the cluster IX family, encoded by mntC. Analysis of an mntC mutant showed that growth of N. gonorrhoeae could be stimulated by addition of either manganese(II) or zinc(II) ions, suggesting that the MntABC system could transport both ions. In contrast, growth of the mntAB mutant in liquid culture was possible only when the medium was supplemented with an antioxidant such as mannitol, consistent with the view that ion transport via MntABC is essential for protection of N. gonorrhoeae against oxidative stress. Using recombinant MntC, we determined that MntC binds Zn(2+) and Mn(2+) with almost equal affinity (dissociation constant of approximately 0.1 microM). Competition assays with the metallochromic zinc indicator 4-(2-pyridylazo)resorcinol showed that MntC binds Mn(2+) and Zn(2+) at the same binding site. Analysis of the N. gonorrhoeae genome showed that MntC is the only Mn/Zn metal binding receptor protein cluster IX in this bacterium, in contrast to the situation in many other bacteria which have systems with dedicated Mn and Zn binding proteins as part of distinctive ABC cassette permeases. Both ...Continue Reading

References

Aug 1, 1979·Journal of General Microbiology·D R VealeH Smith
Jan 1, 1986·Critical Reviews in Microbiology·F Archibald
Jan 1, 1993·Physiological Reviews·B L Vallee, K H Falchuk
Jan 1, 1997·Progress in Biophysics and Molecular Biology·D W Christianson
Feb 27, 1999·Current Opinion in Microbiology·M NaumannT F Meyer
May 14, 1999·Current Opinion in Microbiology·G Storz, J A Imlay
Jul 15, 1999·Nature Structural Biology·Y H LeeC A Hasemann
Oct 14, 2000·Annual Review of Cell and Developmental Biology·A J Merz, M So
Jun 26, 2001·Research in Microbiology·J P Claverys
Jun 21, 2002·Advances in Microbial Physiology·James A Imlay
Jun 1, 1963·Journal of Bacteriology·D S KELLOGGD I PIRKLE
Oct 30, 2003·Journal of Molecular Biology·Soojay BanerjeeThomas J Smith
Jul 17, 2004·Molecular Microbiology·Lauren J McAllisterJames C Paton
Mar 24, 2005·Infection and Immunity·L L GreinerM A Apicella
Apr 22, 2005·Journal of Molecular Biology·Valeria RukhmanNoam Adir
Apr 1, 2006·Molecular Microbiology·Hsing-Ju WuMichael P Jennings
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Kate L SeibAlastair G McEwan

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Citations

Nov 4, 2011·Seminars in Immunopathology·James E Cassat, Eric P Skaar
Jan 17, 2012·Protoplasma·Victoria G LewisChristopher A McDevitt
Feb 28, 1992·Biochimica Et Biophysica Acta·D Gor, J E Mayfield
Apr 17, 2002·Biochimica Et Biophysica Acta·Shirley M Halling, Richard L Zuerner
Mar 21, 2000·Biochimica Et Biophysica Acta·M W Mayo, A S Baldwin
Oct 28, 2010·Antioxidants & Redox Signaling·Melinda J Faulkner, John D Helmann
Jul 3, 2013·Infection and Immunity·Anne JametXavier Nassif
Mar 18, 2009·Infection and Immunity·R Brock Neil, Michael A Apicella
Oct 9, 2012·Journal of Bacteriology·Marie-Christin PawlikUlrich Vogel
Mar 16, 2007·Microbial Cell Factories·Pernilla TurnerEva Nordberg Karlsson
Jun 6, 2009·Future Microbiology·R Brock Neil, Michael A Apicella
Feb 21, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Nan LiQing-Yu He
Dec 31, 2008·Microbes and Infection·R Brock NeilMichael A Apicella
Feb 6, 2015·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Bart A EijkelkampTodd Kitten
Apr 4, 2015·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Jacqueline R MoreyThomas E Kehl-Fie
Mar 19, 2014·Journal of Microbiological Methods·Stacy Sommerfeld RossJennifer Fiegel
Sep 22, 2011·Biochimica Et Biophysica Acta·Claire J Parker SiburtAlvin L Crumbliss
Mar 11, 2015·Structure·Shivani AhujaChristopher M Koth
May 26, 2017·Metallomics : Integrated Biometal Science·Alexander G BobrovRobert D Perry
Oct 19, 2017·Pathogens and Disease·Cynthia Nau Cornelissen
Jul 13, 2018·Frontiers in Cellular and Infection Microbiology·José A GuijarroJessica Méndez
Oct 16, 2019·Journal of Bacteriology·Zhenyao LuoChristopher A McDevitt
Apr 16, 2019·Inorganic Chemistry·Aleksandra HecelHenryk Kozłowski
Sep 8, 2021·Frontiers in Cellular and Infection Microbiology·Marina L ŽupanChristopher A McDevitt

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