PMID: 7015335Feb 1, 1981Paper

Metal cation influence on activity and regulation of aspartate carbamoyltransferase

Proceedings of the National Academy of Sciences of the United States of America
R B HonzatkoW N Lipscomb

Abstract

At saturating carbamoylphosphate and nonsaturating aspartate concentrations, Mg2+, Ca2+, Sr2+, Ba2+, Mn2+, Al3+, and Gd3+ inhibit aspartate carbamoyltransferase (carbamoylphosphate:L-asparate carbamoyltransferase, EC 2.1.3.2) from EScherichia coli. When nucleotide triphosphates are present, these inhibitory effects are displaced to higher concentrations of cation. At lower levels of cation and saturating carbamoylphosphate concentration, Mg2+, Mn2+, Al3+, and Gd3+ partially relieve allosteric inhibition by GTP but have little influence on activation by ATP and inhibition by CTP. At nonsaturating carbamoylphosphate concentrations, however, Mg2+, Mn2+, Al3+, and Gd3+ increase enzymatic activity to 170% over the level when GTP alone is present. In addition, Mg2+, Mn2+, and Al3+ show enhancement of ATP activation by 120-130% but only slight relief of CTP inhibition. We suggest that three modes of action by the metal can account for the observed kinetic behavior. (i) In the absence of nucleotide, metals inhibit catalytic activity either by a direct interaction with the enzyme or indirectly by complexing carbamoylphosphate. (ii) The metal-nucleotide complex interacts allosterically with the enzyme to enhance enzymatic activity relati...Continue Reading

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R B HonzatkoW N Lipscomb
Aug 1, 1972·Biochemistry·R E London, P G Schmidt
Aug 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·M R BethellM E Jones
Jul 13, 1970·Biochemical and Biophysical Research Communications·C C Winlund, M J Chamberlin
Jun 1, 1956·Archives of Biochemistry and Biophysics·R M BOCKS H LIPTON

❮ Previous
Next ❯

Citations

Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R B Honzatko, W N Lipscomb
Jan 1, 1984·The American Journal of Drug and Alcohol Abuse·R Caetano, D Herd
Jan 7, 2016·Chemical Reviews·George P Lisi, J Patrick Loria
Oct 22, 2013·Biochemistry·Gregory M CockrellEvan R Kantrowitz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.