Metal-DNA coordination based bioinspired hybrid nanospheres for in situ amplification and sensing of microRNA.

Journal of Materials Chemistry. B, Materials for Biology and Medicine
Yijing JiaJin Ouyang

Abstract

Sufficient delivery of biomolecules into cells with high loading efficiency and easy cleavability would be significant for the visualization of biomolecules in living cells. Herein, a facile approach based on nano-wire balls (NWs) for efficient loading, intracellular delivery of nucleic acids and in situ targeted miRNA bioimaging is proposed, by feeding of Zn ions for generating DNA-inorganic hybrid structures with large surface areas and good stability. Given that the versatile and robust hybridization chain reaction (HCR) amplification strategy combines DNA assembly with intracellular assay, the resulting NWs without any complicated modification are capable of enhanced signals for the targeted imaging of cancer cells. This method realized a linear detection range of 100 fM to 10 nM, with a low detection limit of 83.6 fM in vitro, and could be used to effectively differentiate the expression levels of miRNA-21 in living cells. Due to its high loading efficiency, excellent biocompatibility and low toxicity, this system can be used to construct a coordination-based delivery nanoplatform for in situ enzyme-free amplified imaging of miRNAs, expanding the application of DNA-based nanomaterials for cellular delivery and intracellula...Continue Reading

References

Jun 2, 2006·Nature Materials·David Putnam
Jun 6, 2009·Journal of the American Chemical Society·Jihyun AnNathaniel L Rosi
Jan 28, 2012·Chemical Reviews·Hong-Cai ZhouOmar M Yaghi
Feb 22, 2012·Science·Shawn M DouglasGeorge M Church
Mar 1, 2012·Nature Materials·Jong Bum LeePaula T Hammond
Aug 6, 2013·Nature Methods·Marshall J LevesqueArjun Raj
Aug 31, 2013·Science·Hiroyasu FurukawaOmar M Yaghi
Feb 24, 2015·Science·Matthew R JonesChad A Mirkin
Apr 1, 2015·Journal of the American Chemical Society·Yulin LiChengde Mao
May 2, 2015·Journal of the American Chemical Society·Zoya CheglakovYossi Weizmann
Sep 4, 2015·Chemical Communications : Chem Comm·Hao LiangJuewen Liu
Feb 2, 2019·Angewandte Chemie·Zhihe QingRonghua Yang
Feb 28, 2019·Analytical Chemistry·Yue LiJinghong Li
Apr 12, 2019·Analytical Chemistry·Zhian HuXinrong Zhang
Jun 22, 2019·Advanced Materials·Hannah J VaughanStephany Y Tzeng
Jul 30, 2019·Advanced Materials·Peng MiKazunori Kataoka
Nov 16, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yiting WangJianhua Wang
Nov 22, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yeling YangZhike He
Dec 18, 2019·Proceedings of the National Academy of Sciences of the United States of America·Maonan WangXuemei Wang
Feb 29, 2020·Chemical Society Reviews·Kaixiang ZhangJinghong Li
Oct 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Caixia HuangRonghua Yang

❮ Previous
Next ❯

Methods Mentioned

BETA
biosensing
biosensor
scanning
transmission electron microscopy
dynamic light scattering
electrophoresis
fluorescence microscopy
fluorescence
confocal microscopy
fluorescence assay

Software Mentioned

OligoAnalyzer

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.