Metal exchange in metallothioneins: a novel structurally significant Cd(5) species in the alpha domain of human metallothionein 1a

The FEBS Journal
Kelly E Rigby DuncanMartin J Stillman

Abstract

Metallothioneins (MTs) are cysteine-rich, metal-binding proteins known to provide protection against cadmium toxicity in mammals. Metal exchange of Zn(2+) ions for Cd(2+) ions in metallothioneins is a critical process for which no mechanistic or structural information is currently available. The recombinant human alpha domain of metallothionein isoform 1a, which encompasses the metal-binding cysteines between Cys33 and Cys60 of the alpha domain of native human metallothionein 1a, was studied. Characteristically this fragment coordinates four Cd(2+) ions to the 11 cysteinyl sulfurs, and is shown to bind an additional Cd(2+) ion to form a novel Cd(5)alpha-MT species. This species is proposed here to represent an intermediate in the metal-exchange mechanism. The ESI mass spectrum shows the appearance of charge state peaks corresponding to a Cd(5)alpha species following addition of 5.0 molar equivalents of Cd(2+) to a solution of Cd(4)alpha-MT. Significantly, the structurally sensitive CD spectrum shows a sharp monophasic peak at 254 nm for the Cd(5)alpha species in contrast to the derivative-shaped spectrum of the Cd(4)alpha-MT species, with peak maxima at 260 nm (+) and 240 nm (-), indicating Cd-induced disruption of the exciton ...Continue Reading

References

Mar 28, 1978·Biochimica Et Biophysica Acta·H Rupp, U Weser
May 31, 1991·Biochemical and Biophysical Research Communications·C Harford, B Sarkar
Sep 1, 1985·The Biochemical Journal·W S Din, J M Frazier
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J D Otvos, I M Armitage
Oct 1, 1982·Journal of Inorganic Biochemistry·Y Boulanger, I M Armitage
Jun 1, 1997·Toxicology and Applied Pharmacology·D Beyersmann, S Hechtenberg
Mar 29, 2007·The FEBS Journal·Kelly E Rigby Duncan, Martin J Stillman

❮ Previous
Next ❯

Citations

Jan 29, 2014·Metallomics : Integrated Biometal Science·Duncan E K Sutherland, Martin J Stillman
Mar 17, 2011·Metallomics : Integrated Biometal Science·Duncan E K Sutherland, Martin J Stillman
Sep 14, 2010·Biochemical and Biophysical Research Communications·Thanh T NguMartin J Stillman
Mar 26, 2009·IUBMB Life·Thanh T Ngu, Martin J Stillman
Jun 6, 2008·Biochemical and Biophysical Research Communications·Duncan E K Sutherland, Martin J Stillman
Jan 30, 2015·Chemical Communications : Chem Comm·Claudia A Blindauer
Dec 2, 2009·Journal of the American Chemical Society·Harini KaluarachchiDeborah B Zamble
Jan 17, 2012·Journal of the American Chemical Society·Duncan E K SutherlandMartin J Stillman
Nov 20, 2015·Metallomics : Integrated Biometal Science·Gordon W IrvineMartin J Stillman
Feb 13, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Judith S SchellerMartin J Stillman
Dec 9, 2017·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Hasan T Imam, Claudia A Blindauer
May 21, 2020·Frontiers in Microbiology·Sergio BalzanoChristophe Brunet
Mar 25, 2010·Biochemistry·Duncan E K SutherlandMartin J Stillman
Nov 3, 2009·Bioorganic & Medicinal Chemistry·Atsushi MiyazakiAkira Takai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of the American Chemical Society
Thanh Ngu, Martin J Stillman
Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
Artur Krezel, Wolfgang Maret
© 2022 Meta ULC. All rights reserved