Metal ion cooperativity in ribozyme cleavage of RNA

Proceedings of the National Academy of Sciences of the United States of America
Mathias Brännvall, L A Kirsebom

Abstract

Combinations of chemical and genetic approaches were used to study the function of divalent metal ions in cleavage of RNA by the ribozyme RNase P RNA. We show that different divalent metal ions have differential effects on cleavage site recognition and rescue of cleavage activity by mixing divalent metal ions that do not promote cleavage by themselves. We conclude that efficient and correct cleavage is the result of cooperativity between divalent metal ions bound at different sites in the RNase P RNA-substrate complex. Complementation of a mutant RNase P RNA phenotype as a result of divalent metal ion replacement is demonstrated also. This finding together with other data indicate that one of the metal ions involved in this cooperativity is positioned near the cleavage site. The possibility that the Mg(2+)/Ca(2+) ratio might regulate the activity of biocatalysts that depend on RNA for activity is discussed.

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Citations

Nov 30, 2002·Biochimie·Mathias BrännvallLeif A Kirsebom
Jul 2, 2003·Current Opinion in Structural Biology·Michael E Harris, Eric L Christian
Sep 19, 2006·Nature Reviews. Microbiology·Alexei V Kazantsev, Norman R Pace
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kristin S KoutmouCarol A Fierke
Jul 31, 2003·Nucleic Acids Research·D Rey BanataoTeri E Klein
Aug 11, 2004·Nucleic Acids Research·Atsushi InoueKazunari Taira
Oct 13, 2004·Nucleic Acids Research·Mathias BrännvallLeif A Kirsebom
May 4, 2005·Nucleic Acids Research·Simona Cuzic, Roland K Hartmann
Dec 8, 2005·Nucleic Acids Research·Ema KikovskaLeif A Kirsebom
Oct 2, 2009·Nucleic Acids Research·Simona Cuzic-FeltensRoland K Hartmann
Nov 22, 2011·Nucleic Acids Research·Ema KikovskaLeif A Kirsebom
Jan 23, 2008·Biological Chemistry·Michal MarszalkowskiRoland K Hartmann
Jul 19, 2008·IUBMB Life·Anastassios VourekasDenis Drainas
Feb 27, 2009·IUBMB Life·Leif A Kirsebom, Stefan Trobro
Jan 13, 2005·Journal of Molecular Biology·Nathan H ZahlerMichael E Harris
Apr 28, 2006·Journal of Molecular Biology·Ema KikovskaLeif A Kirsebom
Jan 27, 2010·Journal of Molecular Biology·William H McClainVenkat Gopalan
Jun 28, 2003·FEMS Microbiology Reviews·David G Kehres, Michael E Maguire
Sep 13, 2016·Journal of Pharmaceutical Sciences·Ravi Shankar Prasad SinghHartmut Derendorf
Apr 16, 2014·Chemical Reviews·W Luke WardVictoria J DeRose
Nov 13, 2019·Chemistry, an Asian Journal·Kristian Le VayHannes Mutschler
Aug 9, 2003·The Journal of Biological Chemistry·Tina PerssonRoland K Hartmann
May 3, 2018·Nucleic Acids Research·Nina M FischerDavid van der Spoel
Oct 8, 2004·Journal of the American Chemical Society·Yasuomi TakagiKazunari Taira

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