Metal ion interaction with urease and UreD-urease apoproteins

Biochemistry
I S Park, Robert P Hausinger

Abstract

Klebsiella aerogenes urease in a Ni-containing enzyme (two Ni per alpha beta gamma unit) that is purified as an apoprotein from cells grown in Ni-free medium. Partial activation of urease and UreD-urease apoproteins is achieved in vitro by incubation in the presence of Ni(II) and CO2, whereas incubation of these proteins with Ni alone leads to the formation of inactive species [Park, I.-S., & Hausinger, R. P. (1995) Science 267, 1156-1158]. Here we determined the kinetics of these inhibitory reactions and demonstrated the presence of two Ni ions per alpha beta gamma unit in the inactive proteins. Although metal-substituted urease has never been purified from Ni-deprived cell, several other metal ions were shown to bind to the urease apoproteins. Divalent Zn, C, Co, and Mn all inhibited Ni- and Co2-promoted urease activation at concentrations below that of Ni, whereas Mg and Ca ions did not inhibit this process. Ni-inhibited species recovered their ability to be partially activated after EDTA treatment. In contrast, samples that were exposed to Co or Cu ions were irreversibly inactivated, and EDTA treatment of Zn- or Mn-inhibited samples led to reduced levels of activation competence. Mn-substituted urease, generated from urease...Continue Reading

References

Aug 1, 1961·The Biochemical Journal·G N WILKINSON

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Citations

Sep 24, 2011·Biochemistry·Eric L CarterRobert P Hausinger
May 31, 2011·Journal of Chemical Information and Modeling·Francesco MusianiStefano Ciurli
Dec 29, 2013·Chemical Reviews·Michael J Maroney, Stefano Ciurli
Jan 5, 2010·Metallomics : Integrated Biometal Science·Eric L CarterRobert P Hausinger
Oct 17, 2006·Journal of Bacteriology·Jong Kyong KimRobert P Hausinger
Oct 4, 2005·Journal of Bacteriology·Jong Kyong KimRobert P Hausinger
Jan 5, 2012·World Journal of Gastroenterology : WJG·Simona CardaropoliTullia Todros
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·A Soriano, R P Hausinger
Oct 16, 2013·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Barbara ZambelliStefano Ciurli
Dec 27, 2011·Journal of Inorganic Biochemistry·Eric L CarterRobert P Hausinger
Oct 1, 2008·Archives of Biochemistry and Biophysics·Soledad Quiroz-ValenzuelaWilliam T Heller
Aug 21, 2007·Phytochemistry·Cristian Follmer
Jul 2, 2014·Biochimica Et Biophysica Acta·Anna MerloniStefano Ciurli
Jun 28, 2003·FEMS Microbiology Reviews·Scott B Mulrooney, Robert P Hausinger
May 5, 1999·Current Opinion in Chemical Biology·M J Maroney
May 12, 2004·Journal of Inorganic Biochemistry·Francesco MusianiStefano Ciurli
Jan 30, 2004·The Journal of Biological Chemistry·Zhenzhan ChangRobert P Hausinger
Nov 20, 2018·Natural Product Reports·Lauren J Rajakovich, Emily P Balskus
Oct 10, 1996·Nature·Z F KanyoD W Christianson
Mar 30, 2013·The Journal of Biological Chemistry·Mark A FarrugiaRobert P Hausinger
Mar 11, 2015·The Journal of Biological Chemistry·Xinming YangHongzhe Sun
Nov 16, 2004·The Journal of Biological Chemistry·Barbara ZambelliStefano Ciurli
Aug 29, 2009·Chemical Reviews·Yanjie Li, Deborah B Zamble
Sep 1, 1996·Journal of Bacteriology·M B Moncrief, R P Hausinger
Nov 7, 1996·Chemical Reviews·William N. Lipscomb, Norbert Sträter
Feb 12, 2004·Chemical Reviews·Jason Kuchar, Robert P Hausinger

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