Metal ions as cofactors for aggregation of therapeutic peptide salmon calcitonin

Inorganic Chemistry
Neeraj RastogiRaja Roy

Abstract

The effects of multivalent metal ions (Cu(2+)/Zn(2+)/Al(3+)) on the aggregation of salmon calcitonin (sCT)--a therapeutic peptide used worldwide in the treatment of osteoporosis and Paget's disease--have been studied in vitro using NMR (both solution state and solid state), TEM, ThT-fluorescence, and FT-IR spectroscopy. Overall, the various results indicated that the metal-ions-induced conformational transitions in the peptide--mostly toward the β-sheet--facilitate the aggregation of sCT in solution. First, the solution NMR has been used to check the interaction between the peptide and the metal ions. Following this, the formation and characterization of calcitonin aggregates has been performed using TEM, solid state NMR, and FT-IR spectroscopy. The TEM and ThT-fluorescence results revealed that the sCT peptide incubated with Cu(2+) and Zn(2+) metal ions (in aqueous environment) forms globular aggregates, while that with Al(3+) ions forms fibrils. The solid state NMR and FT-IR studies revealed the presence of a substantial amount of β-sheet content in sCT aggregates (formed in the presence of these metal ions) compared to the monomeric sCT, indicating that the metal binding is concomitant with conformational changes. The presen...Continue Reading

References

Apr 22, 1986·Biochemistry·R M EpandR L Colescott
Aug 4, 1993·Biochimica Et Biophysica Acta·P J Gilchrist, J P Bradshaw
Dec 1, 1995·Calcified Tissue International·M AzriaJ M Zanelli
Jan 1, 1997·The American Journal of the Medical Sciences·S L Silverman
Mar 10, 1999·Journal of Biomolecular NMR·P AmodeoM A Castiglione Morelli
Jul 20, 1999·European Journal of Clinical Investigation·R D MoirA I Bush
Jun 13, 2000·Protein Science : a Publication of the Protein Society·M KamihiraH Saitô
Sep 6, 2003·Biochimica Et Biophysica Acta·Maria Cristina GaudianoLuisa Valvo
Oct 8, 2003·Current Pharmaceutical Design·N M MehtaJ P Gilligan
Oct 2, 2004·Endocrinology·Ritu KhuranaKunnath P Madhusudan
Jul 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Susan B FowlerJesús Zurdo
Jul 20, 2006·Journal of the American Chemical Society·Liming Hou, Michael G Zagorski
Aug 31, 2006·Biophysical Journal·Marco DiociaiutiMaria Cristina Gaudiano
Jan 9, 2008·Journal of the American Chemical Society·Dror NoyIrit Sagi
Jun 23, 2009·Trends in Pharmacological Sciences·Paolo ZattaStefano L Sensi
Jul 15, 2009·Protein Engineering, Design & Selection : PEDS·Andisheh Abedini, Daniel P Raleigh
Nov 3, 2009·Journal of the American Chemical Society·Sarmad S HindoMi Hee Lim
Nov 6, 2009·Journal of the American Chemical Society·Hai-Young KimMarkus Zweckstetter
Dec 1, 2009·Neuromolecular Medicine·Silvia BologninPaolo Zatta
May 8, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yifat MillerRuth Nussinov
Jun 12, 2010·Journal of the American Chemical Society·Jeffrey R BrenderAyyalusamy Ramamoorthy
May 18, 2011·Biochemical and Biophysical Research Communications·Megan GarveyMarcus Fändrich
May 28, 2011·Journal of Molecular Biology·Samer SalamekhAyyalusamy Ramamoorthy
Jul 2, 2011·Inorganic Chemistry·Daniela ValensinLuigi Messori
Aug 2, 2011·Inorganic Chemistry·Fatima Bousejra-ElGarahChristelle Hureau
Aug 6, 2011·Chemical Society Reviews·Alaina S DeTomaMi Hee Lim
Aug 27, 2011·Metallomics : Integrated Biometal Science·Leonid Breydo, Vladimir N Uversky
Sep 24, 2011·The Journal of Physical Chemistry. B·Kazutoshi YamamotoAyyalusamy Ramamoorthy
Sep 29, 2011·Accounts of Chemical Research·Jeffrey R BrenderAyyalusamy Ramamoorthy
Dec 28, 2011·Journal of Molecular Biology·Rui HuangAyyalusamy Ramamoorthy

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Citations

Dec 5, 2012·Journal of Peptide Science : an Official Publication of the European Peptide Society·Atul Rawat, Dinesh Kumar
Aug 23, 2020·Nitric Oxide : Biology and Chemistry·Huixian YeZhonghong Gao

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