Abstract
In this work, a one-pot method was designed to synthesize copper ions, cadmium ions, lead ions and zinc ions doped chitosan-poly(acrylic acid) nanospheres. Those nanospheres can not only produce independent electrochemical signals, but also react with glutaraldehyde (GA) to immobilize different labeled antibodies. Using the modified nanospheres as immunoprobes, a sandwich-type immunosensor was fabricated to simultaneous detection of four tumor markers (CEA, CA199, CA125 and CA242) of pancreatic cancer. This designed immunosensor exhibited good linear relationships in range from 0.1 to 100ng mL(-1) for CEA, 1 to 150UmL(-1) for CA199, CA125 and CA242, corresponding detection limits 0.02ng mL(-1), 0.4UmL(-1), 0.3UmL(-1) and 0.4UmL(-1), respectively. Meanwhile, the immunosensor was applied in analysis of clinical serum samples, whose results were well agreed with the enzyme-linked immunosorbent assay (ELISA), indicating that the proposed immunosensor gave a hope for the identification and validation of specific early cancer.
References
Apr 20, 2001·Biomaterials·J S AhnC S Cho
Jul 10, 2002·Biomaterials·Yong HuChangzheng Yang
Apr 3, 2003·Nature Reviews. Cancer·Julia D WulfkuhleEmanuel F Petricoin
Apr 13, 2007·Biomaterials·Yang YangJunbai Li
Jul 29, 2011·Chemical Society Reviews·Maëlle PerfézouArben Merkoçi
Jul 28, 2012·ACS Nano·Bhaskara V ChikkaveeraiahXiaoyuan Chen
Nov 22, 2012·Chemical Communications : Chem Comm·Fen-Ying KongHong-Yuan Chen
Jul 17, 2013·CA: a Cancer Journal for Clinicians·Christopher L WolfgangRalph H Hruban
Feb 20, 2014·The Analyst·Yaping ZhangZhili Li
Citations
Oct 16, 2016·Scientific Reports·Zhongxue Tang, Zhanfang Ma
Apr 28, 2017·Sensors·Paloma Yáñez-SedeñoJosé M Pingarrón
Apr 8, 2017·Sensors·Benoît Piro, Steeve Reisberg
Feb 20, 2017·Biosensors & Bioelectronics·Xiaoqiang LiuHuangxian Ju
May 4, 2018·Expert Review of Molecular Diagnostics·Shuang Yin, Zhanfang Ma
May 18, 2016·The Analyst·Hui ChengGuosong Lai
May 14, 2019·Electrophoresis·Yu Jiang, Jayne Wu
Jun 6, 2019·The Analyst·Lei YuDazhong Shen
Feb 15, 2020·IUBMB Life·Fatemeh SadoughiSeyyed Mehdi Mirhashemi
Jun 7, 2020·Micromachines·Wen-Chi YangCongo Tak-Shing Ching
Sep 22, 2019·Nanomaterials·Xin DuMin Liu
Nov 11, 2018·Environmental Science and Pollution Research International·Fabiane Grecco da Silva PortoIrene Teresinha Santos Garcia
Apr 29, 2019·Mikrochimica Acta·Thitirat PutninRawiwan Laocharoensuk
Mar 30, 2018·Mikrochimica Acta·Mei ChenJiali Tang
Mar 6, 2021·Analytical and Bioanalytical Chemistry·Wenxian ZhangChonghai Xu
Jul 20, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Kulrisa KuntamungKontad Ounnunkad
Sep 3, 2021·Biosensors & Bioelectronics·Xuejiao LiuWensheng Yang