Metal Ions Induce Liquid Condensate Formation by the F Domain of Aedes aegypti Ecdysteroid Receptor. New Perspectives of Nuclear Receptor Studies.

Anna WiȩchMarek Orłowski


The superfamily of nuclear receptors (NRs), composed of ligand-activated transcription factors, is responsible for gene expression as a reaction to physiological and environmental changes. Transcriptional machinery may require phase separation to fulfil its role. Although NRs have a similar canonical structure, their C-terminal domains (F domains) are considered the least conserved and known regions. This article focuses on the peculiar molecular properties of the intrinsically disordered F domain of the ecdysteroid receptor from the Aedes aegypti mosquito (AaFEcR), the vector of the world's most devastating human diseases such as dengue and Zika. The His-Pro-rich segment of AaFEcR was recently shown to form the unique poly-proline helix II (PPII) in the presence of Cu2+. Here, using widefield microscopy of fluorescently labeled AaFEcR, Zn2+- and Cu2+-induced liquid-liquid phase separation (LLPS) was observed for the first time for the members of NRs. The perspectives of this finding on future research on the F domain are discussed, especially in relation to other NR members.


May 1, 1969·The American Journal of Tropical Medicine and Hygiene·N J MarchetteA Rudnick
Jun 28, 2001·Physiological Reviews·A Aranda, A Pascual
Nov 10, 2001·Trends in Endocrinology and Metabolism : TEM·L Altucci, H Gronemeyer
Mar 23, 2002·Protein Science : a Publication of the Protein Society·Vladimir N Uversky
Aug 2, 2002·The Lancet Infectious Diseases·Lucia Malaguarnera, Salvatore Musumeci
Sep 13, 2002·Insect Biochemistry and Molecular Biology·Alexander S RaikhelGeoff Attardo
Sep 1, 1952·Transactions of the Royal Society of Tropical Medicine and Hygiene·G W A DICKA J HADDOW
Mar 30, 2004·Proteins·Joshua M Hicks, Victor L Hsu
Jun 1, 2006·Biochemistry·Jiangang LiuA Keith Dunker
Aug 18, 2006·Annual Review of Entomology·Alan D T Barrett, Stephen Higgs
Jan 25, 2008·BMC Bioinformatics·Yang Zhang
Jan 22, 2009·Proteins·Małgorzata Nocula-ŁugowskaAndrzej Ozyhar
Jul 7, 2009·Annual Review of Microbiology·Ahmed S I AlyStefan H I Kappe
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jayme A Souza-NetoGeorge Dimopoulos
Feb 13, 2010·Annual Review of Physiology·Pengxiang HuangFraydoon Rastinejad
Jul 27, 2010·Advanced Drug Delivery Reviews·Ira G Schulman
Oct 5, 2010·Current Opinion in Pharmacology·A Keith Dunker, Vladimir N Uversky
Nov 11, 2010·Proteins·Agnieszka Dziedzic-LetkaAndrzej Ożyhar
Nov 17, 2010·Nature Reviews. Microbiology·Maria G GuzmanRosanna W Peeling
Jan 5, 2011·Biochimica Et Biophysica Acta·Mads M AagaardSusanne Mandrup
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
Jan 17, 2012·Current Topics in Medicinal Chemistry·Michal PawlakBart Staels
Mar 13, 2012·Trends in Microbiology·James P White, Richard E Lloyd
Aug 11, 2012·PLoS Neglected Tropical Diseases·Oliver J BradySimon I Hay
Mar 20, 2013·Journal of Molecular Biology·Alexei A AdzhubeiAlexander A Makarov
Apr 9, 2013·Nature·Samir BhattSimon I Hay
Aug 31, 2013·Journal of Innate Immunity·April M ClaytonGeorge Dimopoulos
Oct 10, 2013·Journal of Molecular Endocrinology·Fraydoon RastinejadSepideh Khorasanizadeh
Nov 29, 2013·Molecular Endocrinology·S Stoney SimonsRaj Kumar
Jan 15, 2014·PLoS Neglected Tropical Diseases·Oumar FayeAmadou Alpha Sall
Oct 8, 2014·Annual Review of Cell and Developmental Biology·Anthony A HymanFrank Jülicher
Jul 18, 2015·Molecular and Cellular Endocrinology·Shivali R Patel, Debra F Skafar
Sep 22, 2015·Nature Materials·Felipe García Quiroz, Ashutosh Chilkoti
Jan 6, 2016·Cell Communication and Signaling : CCS·Diana M Mitrea, Richard W Kriwacki
Jan 6, 2016·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·A S Oliveira MeloA M Bispo de Filippis
Mar 18, 2016·Current Opinion in Virology·Konstantin A TsetsarkinScott C Weaver
Jun 13, 2016·Trends in Cell Biology·David S W Protter, Roy Parker

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