Metal-Organic Framework Disintegrants: Enzyme Preparation Platforms with Boosted Activity.

Angewandte Chemie
Hongde AnYao Chen

Abstract

An enzyme formulation using customized enzyme activators (metal ions) to directly construct metal-organic frameworks (MOFs) as enzyme protective carriers is presented. These MOF carriers can also serve as the disintegrating agents to simultaneously release enzymes and their activators during biocatalysis with boosted activities. This highly efficient enzyme preparation combines enzyme immobilization (enhanced stability, easy operation) and homogeneous biocatalysis (fast diffusion, high activity). The MOF serves as an ion pump that continuously provides metal ion activators that greatly promote the enzymatic activities (up to 251 %). This MOF-enzyme composite demonstrated an excellent protective effect against various perturbation environments. A mechanistic investigation revealed that the spontaneous activator/enzyme release and ion pumping enable enzymes to sufficiently interact with their activators owing to the proximity effects, leading to a boost in biocatalytic performance.

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Citations

Apr 25, 2021·Advanced Materials·Weiqing XuChengzhou Zhu
Sep 16, 2021·Chemical Communications : Chem Comm·Chen XuXiang Ma
Nov 24, 2021·ACS Applied Materials & Interfaces·Cuie Wang, Kaiming Liao
Dec 21, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Hongtai ChenXiutang Zhang
Jan 4, 2022·ACS Applied Materials & Interfaces·Man ZhangYu-Kui Zhang
Jan 12, 2022·Preparative Biochemistry & Biotechnology·Yuefeng DengKefeng Ma

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