Metalloproteinase-9 mRNA expression in monocytes from patients with chronic renal failure

American Journal of Nephrology
I EbiharaH Koide

Abstract

Long-term dialysis patients suffer from various complications including atherosclerosis. It has been suggested that metalloproteinases (MMPs) contribute to vascular remodeling during the development and progression of human atherosclerosis. Activated human monocytes have been demonstrated to secrete MMPs. In the present study, we measured levels of MMP mRNA in peripheral blood monocytes obtained from patients on continuous ambulatory peritoneal dialysis (CAPD) or hemodialysis (HD) and chronic-renal-failure patients not undergoing dialysis. Twenty patients with chronic renal failure were not undergoing dialysis, 20 patients were on CAPD, 40 patients were on chronic HD and 20 healthy volunteers served as controls. We used cDNA probes encoding for MMP-1, MMP-2, MMP-3 and MMP-9 and glyceraldehyde phosphate dehydrogenase. Higher levels of MMP-9 mRNA in the peripheral blood monocytes were observed in HD patients than in CAPD patients, undialyzed chronic renal failure patients or healthy controls. MMP-9 mRNA levels at the end of HD were not significantly higher than those at the start of HD. MMP-9 mRNA levels from HD patients did not differ among the types of membranes. We could detect minimal MMP-1, MMP-2 and MMP-3 mRNA expression in...Continue Reading

Citations

Oct 9, 1998·Clinica Chimica Acta; International Journal of Clinical Chemistry·T NakamuraH Koide
Sep 25, 2002·Clinical Biochemistry·Fen-Pi ChouYih-Shou Hsieh
Mar 13, 2003·Kidney International·Kathyrn D RodgersDominic Cosgrove
Jan 24, 2004·The Journal of Dermatology·Girish MunavalliJack L Arbiser
Sep 27, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Bernardo P MarsonJose E Tanus-Santos
Feb 21, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Amanda K LeoneJonathan M King
Nov 7, 2012·American Journal of Nephrology·Rieko OkadaUNKNOWN Japan Multi-Institutional Collaborative Cohort (J-MICC) Study Group
Jun 24, 2003·Journal of the American Society of Nephrology : JASN·Peter StenvinkelBengt Lindholm

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