Metalloproteinase and cytokine production by THP-1 macrophages following exposure to chitosan-DNA nanoparticles

Biomaterials
Fatiha ChellatDominique Laurent-Maquin

Abstract

The use of nanoparticles for gene therapy is gaining more and more interest for medical applications. Chitosan is among the candidate polymers that have a potential application as a gene delivery system. Before using chitosan-DNA nanoparticles in vivo, one must study their interaction and cell's behavior. Since macrophages play an important role in inflammatory processes, this study was performed to investigate the effects of chitosan-DNA nanoparticles on human THP-1 cell line. Cytokine (TNF-alpha, IL-1beta, IL-6 and IL-10) and metalloproteinase (MMP-2 and MMP-9) release as well as their inhibitors (TIMP-1 and TIMP-2) were assessed after time course incubation with different amount of nanoparticles. Their secretion was quantified by enzyme-linked immunosorbent assay. Gelatinolytic activity of MMP-2 and MMP-9 was determined by zymography in cell supernatants and lysates. Cytokine secretion was not detected even in the presence of high amount of nanoparticles. On the contrary, the secretion of MMP-9 in cell supernatants increased significantly after 24 and 48 h in comparison with non-treated cells. MMP-2 secretion was augmented only after 48 h for the highest concentrations of nanoparticles (10 and 20 microg/ml DNA content). Howe...Continue Reading

References

Jan 1, 1990·Biomaterials, Artificial Cells, and Artificial Organs·T Chandy, C P Sharma
Jan 1, 1995·Bioconjugate Chemistry·A V Kabanov, V A Kabanov
May 31, 1994·Annals of the New York Academy of Sciences·K Boris-Lawrie, H M Temin
Jan 1, 1996·Clinical & Experimental Metastasis·C J SwallowJ G Guillem
May 2, 1996·The New England Journal of Medicine·J M Wilson
Jun 1, 1996·Current Opinion in Structural Biology·V A Bloomfield
Dec 10, 1996·AIDS Research and Human Retroviruses·R Löbenberg, J Kreuter
Aug 28, 1998·Human Gene Therapy·V L Truong-LeK W Leong
Nov 5, 1998·Journal of Controlled Release : Official Journal of the Controlled Release Society·F C MacLaughlinA P Rolland
May 26, 1999·American Journal of Respiratory Cell and Molecular Biology·D F GibbsJ Varani
Mar 4, 2000·International Journal of Pharmaceutics·C KneuerC M Lehr
Aug 5, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·C E SomaP Couvreur
Feb 22, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·H Q MaoK W Leong
Nov 24, 2001·Advanced Drug Delivery Reviews·G Borchard
Jan 29, 2002·European Journal of Immunology·Stephen C RobinsonFrances R Balkwill

❮ Previous
Next ❯

Citations

Nov 21, 2008·Journal of Materials Science. Materials in Medicine·L X LiuX G Leng
Dec 30, 2008·Journal of Materials Science. Materials in Medicine·V K Mourya, Nazma N Inamdar
Aug 14, 2013·Tissue Engineering. Part a·Joana MacielMário A Barbosa
Jul 2, 2011·International Journal of Nanomedicine·Yan GaoXiao-Rong Li
Jun 1, 2013·International Journal of Nanomedicine·Meenakshi MalhotraSatya Prakash
Aug 29, 2007·International Journal of Nanomedicine·Katherine Bowman, Kam W Leong
Mar 21, 2007·International Journal of Nanomedicine·Hui DaiHai-Quan Mao
Jun 10, 2010·Nanomedicine·Roberta CortivoBarbara Zavan
Jun 1, 2014·Journal of Materials Science. Materials in Medicine·Andrea NolteHans Peter Wendel
Mar 29, 2014·Nanomedicine·Sara VicenteMaria J Alonso
Jan 12, 2011·Expert Opinion on Drug Delivery·Avnesh Kumari, Sudesh Kumar Yadav
Jun 12, 2009·Drug Development and Industrial Pharmacy·Meenakshi MalhotraSatya Prakash
Apr 30, 2013·Methods : a Companion to Methods in Enzymology·Mehfuz ZamanIstvan Toth
Jan 18, 2016·Journal of Nanobiotechnology·Rajneesh ChaudharyJagat Rakesh Kanwar
Jun 1, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jung-hua Steven KuoYi-lin Lin
Sep 15, 2005·British Journal of Pharmacology·Anna RadomskiMarek W Radomski
May 18, 2007·Journal of Biomedical Materials Research. Part a·A J AsikainenR Suuronen
Aug 10, 2007·Journal of Biomedical Materials Research. Part a·Ximing XuMyron Spector
Nov 26, 2010·Journal of Biomedical Materials Research. Part a·Lanxia LiuXigang Leng
Sep 29, 2011·Journal of Biomedical Materials Research. Part a·Y M ThasneemChandra P Sharma
Apr 25, 2013·Journal of Biomedical Materials Research. Part a·Ragima NadeshDeepthy Menon
May 3, 2006·Biomaterials·Arnaud VonarbourgJean-Pierre Benoit
Dec 31, 2005·Journal of Bioscience and Bioengineering·Ken SasakiNapavarn Noparatnaraporn
Jul 19, 2005·Biomaterials·Fatiha ChellatL'Hocine Yahia
Nov 7, 2013·International Journal of Biomaterials·Haliza KatasKai Leong Lam
May 28, 2015·Acta Biomaterialia·Mithun Varghese VadakkanG S Vinod Kumar
Dec 3, 2014·International Journal of Pharmaceutics·Nadège GrabowskiElias Fattal
Jun 12, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Purna Sai KorrapatiSeeram Ramakrishna
Oct 25, 2016·International Journal of Pharmaceutics·Gizem BorGülşah Şanlı-Mohamed
Oct 1, 2016·Bio-medical Materials and Engineering·Markus DenzingerStefanie Krajewski
Apr 16, 2016·Annals of Biomedical Engineering·Luke D Amer, Stephanie J Bryant
Sep 20, 2018·Current Drug Delivery·Srijita ChakrabartiBhaskar Mazumder
Jan 12, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Petra Lynen JansenPeter R Mertens
Oct 7, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Huei-Wen ChenPan-Chyr Yang
Dec 13, 2019·Future Science OA·Manuela Giraldo-VillegasMauricio Rojas-López
Jan 19, 2021·Chemical Biology & Drug Design·Nafiseh KhelghatiBahman Yousefi
Mar 7, 2009·Molecular Pharmaceutics·Aditya PattaniPravin D Potdar

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