PMID: 8595129Nov 1, 1995Paper

Metalloproteinase secretion by endometrial leukocyte subsets

American Journal of Reproductive Immunology : AJRI
W ShiP Bischof

Abstract

Human endometrium contains up to 40% leukocytes. Since their function is unclear, we cultured these cells separately to analyze their proteolytic potential in vitro. Endometrium was obtained from hysterectomies or from first trimester abortion. After collagenase digestion, the different leukocyte subsets (CD56, CD3, CD19, CD16, CD45) were immuno-separated and cultured. Gelatine zymography and a quantitative type IV gelatinase assay were run on all culture supernatants. On zymography, all subtypes of endometrial leukocytes released gelatinases. The 72-kDa gelatinase was absent from peripheral leukocyte supernatants, whereas it was secreted by all endometrial leukocytes. The 92-kDa gelatinase was present in all culture supernatants. Large granular lymphocytes (CD56+, LGL) expressed the highest type VI gelatinase activity as compared to all other leukocytes. This activity increased during the cycle in LGL and T cells but remained unchanged in glandular and stromal cells. Endometrial leukocytes, particularly LGL, secrete significant amounts of gelatinases.

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Citations

Oct 26, 1999·Journal of Reproductive Immunology·L A Salamonsen, D E Woolley
Apr 13, 2002·BJOG : an International Journal of Obstetrics and Gynaecology·Simon JacksonPaul Abrams
Oct 1, 1994·American Journal of Reproductive Immunology : AJRI·C A WitzR S Schenken
Apr 19, 2016·American Journal of Human Biology : the Official Journal of the Human Biology Council·Judyta NowakBoguslaw Pawlowski

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