Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse

Mediators of Inflammation
Ann Kristin BartonHeidrun Gehlen

Abstract

In chronic respiratory disease, matrix metalloproteinases (MMPs) contribute to pathological tissue destruction when expressed in excess, while tissue inhibitors of metalloproteinases (TIMPs) counteract MMPs with overexpression leading to fibrosis formation. They may be out of balance in equine pneumopathies and serve as biomarkers of pulmonary inflammation. We hypothesized that MMPs and TIMPs correlate to clinical findings and bronchoalveolar lavage fluid cytology in different equine chronic pneumopathies. Using a scoring system, 61 horses were classified controls as free of respiratory disease (n = 15), recurrent airway obstruction (RAO, n = 17), inflammatory airway disease (IAD, n = 18), or chronic interstitial pneumopathy (CIP, n = 11). Zymography and equine MMP and TIMP assays were used to detect MMP-2, MMP-8, MMP-9 as well as TIMP-1, and TIMP-2 in BALF supernatant. MMP-2, TIMP-1, and TIMP-2 concentrations were significantly increased in RAO and IAD compared to controls. MMP-9 concentration and MMP-8 activity evaluated by fluorimetry were significantly increased in RAO, IAD, and CIP. These results were confirmed by zymography for MMP-2 and MMP-9 activity in 52 horses. In conclusion, MMPs and TIMPs correlate well with clinic...Continue Reading

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Citations

Jan 6, 2017·Mediators of Inflammation·Ann Kristin Barton, Heidrun Gehlen
Sep 10, 2020·Frontiers in Veterinary Science·Laurent CouetilCyprianna Swiderski
Jan 26, 2021·Mediators of Inflammation·Ann Kristin BartonHeidrun Gehlen
Sep 2, 2021·Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere·Ann Kristin Barton, Heidrun Gehlen

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Methods Mentioned

BETA
Bronchoalveolar Lavage
electrophoresis
ELISAs
ELISA
zymography
biopsies

Software Mentioned

ImageJ
Excel
SPSS

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