Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegans

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
Natasa PolakStephen R Stürzenbaum

Abstract

Zinc oxide nanoparticles (ZnONPs) are used in large quantities by the cosmetic, food and textile industries. Here we exposed Caenorhabditis elegans wild-type and a metal sensitive triple knockout mutant (mtl-1;mtl-2;pcs-1) to ZnONPs (0-50mg/L) to study strain and exposure specific effects on transcription, reactive oxygen species generation, the biomolecular phenotype (measured by Raman microspectroscopy) and key endpoints of the nematode life cycle (growth, reproduction and lifespan). A significant dissolution effect was observed, where dissolved ZnO constituted over 50% of total Zn within a two day exposure to the test medium, suggesting that the nominal exposure to pure ZnONPs represents in vivo, at best, a mixture exposure of ionic zinc and nanoparticles. Nevertheless, the analyses provided evidence that the metallothioneins (mtl-1 and mtl-2), the phytochelatin synthase (pcs-1) and an apoptotic marker (cep-1) were transcriptionally activated. In addition, the DCFH-DA assay provided in vitro evidence of the oxidative potential of ZnONPs in the metal exposure sensitive triple mutant. Raman spectroscopy highlighted that the biomolecular phenotype changes significantly in the mtl-1;mtl-2;pcs-1 triple knockout worm upon ZnONP ex...Continue Reading

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Citations

Jul 9, 2016·Environmental Toxicology and Pharmacology·Soudabeh ImanikiaVolker M Arlt
Jul 2, 2014·Human & Experimental Toxicology·H HsiehM B Genter
Jul 5, 2017·Nanotoxicology·Laura Gonzalez-MoragasAnna Laromaine
Feb 26, 2016·International Journal of Molecular Sciences·Yona J EssigStephen R Stürzenbaum
Aug 21, 2020·Environmental Science and Pollution Research International·Graziella FicocielloAndrea Setini
Jan 13, 2021·Nanomaterials·Tung X Trinh, Jongwoon Kim
Mar 22, 2021·The Science of the Total Environment·Qiang ChuXiaodong Zheng

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