Metalloregulatory properties of the ArsD repressor.

The Journal of Biological Chemistry
Y Chen, Barry P Rosen

Abstract

The plasmid-encoded arsenical resistance (ars) operon of plasmid R773 produces resistance to trivalent and pentavalent salts of the metalloids arsenic and antimony in cells of Escherichia coli. The first two genes in the operon, arsR and arsD, were previously shown to encode trans-acting repressor proteins. ArsR controls the basal level of expression of the operon, while ArsD controls maximal expression. Thus, action of the two repressors form a homeostatic regulatory circuit that maintains the level of ars expression within a narrow range. In this study, we demonstrate that ArsD binds to the same site on the ars promoter element as ArsR but with 2 orders of magnitude lower affinity. The results of gel shift assays demonstrate that ArsD is released from the ars DNA promoter by phenylarsine oxide, sodium arsenite, and potassium antimonyl tartrate (in order of effectiveness), the same inducers to which ArsR responds. Using the quenching of intrinsic tryptophan fluorescence to measure the affinity of the repressor for inducers, apparent Kd values for Sb(III) and As(III) of 2 and 60 microM, respectively, were obtained. These results demonstrate that the arsR-arsD pair provide a sensitive mechanism for sensing a wide range of enviro...Continue Reading

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Citations

Dec 13, 2006·Archives of Microbiology·Prerna C PatelJonathan R Lloyd
Oct 24, 2007·Journal of Bioenergetics and Biomembranes·Yung-Feng LinBarry P Rosen
Aug 2, 2002·Plasmid·David Ryan, Emer Colleran
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May 18, 2006·Annual Review of Microbiology·John F StolzRonald S Oremland
Oct 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yung-Feng LinBarry P Rosen
Dec 29, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·A Abdul AjeesBarry P Rosen
Nov 14, 2013·Journal of Environmental Sciences (China)·Wei ZhengRen Zhang
Oct 27, 2007·Protein Science : a Publication of the Protein Society·Ivan I VorontsovWayne F Anderson
Jun 14, 2013·Environmental Microbiology Reports·Djamila Slyemi, Violaine Bonnefoy
Jan 16, 2013·Microbial Biotechnology·Davide MerullaJan Roelof van der Meer
Mar 29, 2014·Environmental Microbiology·Yoon-Suk KangTimothy R McDermott
Dec 17, 2008·FEMS Microbiology Reviews·Catarina L SantosPhilippe Normand
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Apr 3, 2016·Applied and Environmental Microbiology·Yoon-Suk KangTimothy R McDermott
Aug 12, 2014·Applied and Environmental Microbiology·Danielle MoinierViolaine Bonnefoy
Apr 19, 2007·The Journal of Biological Chemistry·Yung-Feng LinBarry P Rosen
Dec 18, 2018·Frontiers in Microbiology·Tomislav CernavaGabriele Berg
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Aug 1, 2009·EcoSal Plus·Dietrich H Nies, Gregor Grass
Aug 14, 2021·Molecular Microbiology·Erin E PriceJeffrey M Boyd
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