Metallothionein gene activation in the earthworm (Lumbricus rubellus)

Biochemical and Biophysical Research Communications
M HöcknerS R Stürzenbaum

Abstract

In order to cope with changing environmental conditions, organisms require highly responsive stress mechanisms. Heavy metal stress is handled by metallothioneins (MTs), the regulation of which is evolutionary conserved in insects and vertebrates and involves the binding of metal transcription factor 1 (MTF-1) to metal responsive elements (MREs) positioned in the promoter of MT genes. However, in most invertebrate phyla, the transcriptional activation of MTs is different and the exact mechanism is still unknown. Interestingly, although MREs are typically present also in invertebrate MT gene promoters, MTF-1 is notably absent. Here we use Lumbricus rubellus, the red earthworm, to study the elusive mechanism of wMT-2 activation in control and Cd-exposed conditions. EMSA and DNase I footprinting approaches were used to pinpoint functional binding sites within the wMT-2 promoter region, which revealed that the cAMP responsive element (CRE) is a promising candidate which may act as a transcriptional activator of invertebrate MTs.

References

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Citations

Jan 8, 2016·International Journal of Molecular Sciences·Gregory R KowaldClaudia A Blindauer
Jan 26, 2016·International Journal of Molecular Sciences·Adolf Michael Sandbichler, Martina Höckner
Oct 5, 2018·Metallomics : Integrated Biometal Science·Sara CalatayudRicard Albalat
Oct 2, 2019·Molecular Biology Reports·Victoria DrechselMartina Höckner
May 26, 2017·International Journal of Molecular Sciences·Victoria DrechselMartina Höckner
Jan 23, 2020·International Journal of Molecular Sciences·Martina HöcknerLars Tomanek

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Methods Mentioned

BETA
PCR
footprinting

Software Mentioned

TRANSFAC
TRANSFAC TF
Peakscanner

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