Metallothionein (MT) -I and MT-II expression are induced and cause zinc sequestration in the liver after brain injury.

PloS One
Michael W PankhurstRoger S Chung

Abstract

Experiments with transgenic over-expressing, and null mutant mice have determined that metallothionein-I and -II (MT-I/II) are protective after brain injury. MT-I/II is primarily a zinc-binding protein and it is not known how it provides neuroprotection to the injured brain or where MT-I/II acts to have its effects. MT-I/II is often expressed in the liver under stressful conditions but to date, measurement of MT-I/II expression after brain injury has focused primarily on the injured brain itself. In the present study we measured MT-I/II expression in the liver of mice after cryolesion brain injury by quantitative reverse-transcriptase PCR (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) with the UC1MT antibody. Displacement curves constructed using MT-I/II knockout (MT-I/II(-/-)) mouse tissues were used to validate the ELISA. Hepatic MT-I and MT-II mRNA levels were significantly increased within 24 hours of brain injury but hepatic MT-I/II protein levels were not significantly increased until 3 days post injury (DPI) and were maximal at the end of the experimental period, 7 DPI. Hepatic zinc content was measured by atomic absorption spectroscopy and was found to decrease at 1 and 3 DPI but returned to normal by 7DPI. Zinc...Continue Reading

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Aug 16, 2013·Current Opinion in Clinical Nutrition and Metabolic Care·Deborah R Morris, Cathy W Levenson
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Methods Mentioned

BETA
transgenic
PCR
enzyme-linked immunosorbent assay
biopsy
dissection
biopsies
ELISA
Assay
atomic absorption spectroscopy

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