Metallothioneins and heat shock proteins 70 in Armadillidium vulgare (Isopoda, Oniscidea) exposed to cadmium and lead

Ecotoxicology and Environmental Safety
Veronica MazzeiGuglielmo Longo

Abstract

The heavy metals bioaccumulation capability in Armadillidium vulgare feeded with chestnut leaves contaminated with various sublethal concentrations of Cd and Pb, was evaluated under laboratory conditions. The metal concentration found in the hepatopancreas of treated animals, as measured by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), affected the expression and localization of MT and HSP70 as shown by immunohistochemical and western blotting analysis. The Cd content of the animals treated with the various concentrations of the metal has been always higher than that of chestnut leaves contaminated. The accumulation of Pb was, instead, always modest compared to the content of the chestnut leaves. The immunohistochemical investigation in hepatopancreas tissue of animals treated with increasing concentrations of Cd and Pb, by using the anti-MT and anti-HSP70 antibodies, has provided a response clearly positive even if differentiated in relation to the metal and concentration tested. In particular, a positive response to anti-MT antibody was detected in B and S cells nuclei and S cells cytoplasm; the localization of HSP70 was particularly intense at the cell surface. Western blotting analysis showed significant up-regulat...Continue Reading

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Citations

Dec 20, 2018·ZooKeys·Cornelis A M van GestelPrimož Idar
Mar 15, 2018·Frontiers in Endocrinology·Éverton L VogtAnapaula S Vinagre
Jan 15, 2019·Microscopy Research and Technique·Angeliki DroutsaBianca Maria Lombardo
Nov 30, 2019·Microscopy Research and Technique·Giulia FerraraMaria Violetta Brundo
May 29, 2020·Ecotoxicology and Environmental Safety·Elena Maria ScalisiMaria Violetta Brundo

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