Metamorphosis inhibition: an alternative rearing protocol for the newt, Cynops pyrrhogaster

Zoological Science
Chikafumi ChibaMiyako Takayanagi

Abstract

The newt is an indispensable model animal, of particular utility for regeneration studies. Recently, a high-throughput transgenic protocol was established for the Japanese common newt, Cynops pyrrhogaster. For studies of regeneration, metamorphosed animals may be favorable; however, for this species, there is no efficient protocol for maintaining juveniles after metamorphosis in the laboratory. In these animals, survival drops drastically after metamorphosis as their foraging behaviour changes to adapt to a terrestrial habitat, making feeding in the laboratory with live or moving foods more difficult. To elevate the efficiency of laboratory rearing of this species, we examined metamorphosis inhibition (Ml) protocols to bypass the period (four months to two years after hatching) in which the animal feeds exclusively on moving foods. We found that approximately 30% of animals survived after 2-year Ml, and that the survivors continuously grew, only with static food while maintaining their larval form and foraging behaviour in 0.02% thiourea (TU) aqueous solution, then metamorphosed when returned to a standard rearing solution even after 2-year-MI. The morphology and foraging behavior (feeding on static foods in water) of these met...Continue Reading

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Apr 30, 2011·Nature Protocols·Martin Miguel Casco-RoblesChikafumi Chiba

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Citations

Jun 17, 2021·Development, Growth & Differentiation·Mitsuki KyakunoToshinori Hayashi

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Methods Mentioned

BETA
transgenic
amputation

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